拟南芥AtHHR3响应热胁迫应答的初步分析
Primary Analysis of the Function of Arabidopsis thaliana AtHHR3 to Response Heat Stress
作者：齐辉辉(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；蔡潇潇(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；杨虹伟(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；王健美(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；刘志斌(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；杨 毅(四川大学生命科学学院生物资源与生态环境教育部重点实验室)
Author：QI Hui-Hui(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；CAI Xiao-Xiao(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；YANG Hong-Wei(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；WANG Jian-Mei(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；LIU Zhi-Bin(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；YANG Yi(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)
收稿日期：2015-12-31 年卷（期）页码：2016,53(5):1141-1146
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：AtHHR3; E3连接酶; 热胁迫; 拟南芥; 耐热性
Key words：AtHHR3; E3 ligase; heat stress; Arabidopsis thaliana; thermotolerance
基金项目：
中文摘要
E3连接酶在植物响应各种生物和非生物胁迫中起到非常重要的作用。拟南芥中大约含有1400多种E3连接酶，然而，到目前为止对于E3连接酶在植物响应热胁迫中的功能所知甚少。拟南芥基因AtHHR3是一个RING结构域的E3连接酶，通过生物信息学分析发现其可能参与植物热胁迫相关的应答。为了探索其具体的功能，构建了AtHHR3互补株系，并在DNA水平和转录水平分别鉴定了AtHHR3互补株系，用RT-PCR技术分析了AtHHR3在热处理条件下基因表达的变化情况。在热胁迫下分析了野生型、突变体athhr3、回复株系幼苗存活以及种子萌发的表型变化情况，发现突变体athhr3表现出对热胁迫的耐受性，并检测了热胁迫下不同株系的HSF、HSP等热相关基因的转录水平的变化，初步的研究表明拟南芥基因AtHHR3负调控植物对热胁迫的耐受性。
英文摘要
E3 ligase is very important for plant survival when plants confronted with variety biotic and abiotic stresses. There are more than 1400 E3 ligases in Arabidopsis thaliana. However, little is known about the function of E3 ligases in plants response to heat stress so far. Arabidopsis thaliana gene AtHHR3 encodes a RING domain E3 ligase. According to the analysis in silico, it was found that the gene might participate in Arabidopsis thaliana heat stress response. In order to explore the detail function of AtHHR3, we constructed the transgene lines of AtHHR3 which was identified in DNA level and the transcription level. We analyzed the changes of AtHHR3 gene expression by RT-PCR under different temperature. Our analysis demonstrated that athhr3 mutant plants had a higher germination rate and survival rate than wild type (col) and AtHHR3 transgenic lines under heat stress, suggesting that athhr3 mutant plants were more tolerant than wild type and AtHHR3 transgenic lines to heat shock. The changes of transcription of some HSFs and HSPs were detected in different strains under heat stress. Hence, our preliminary studies reveal that AtHHR3 acts as a negative regulator of thermotolerance in Arabidopsis thaliana.
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