For accurate identification of three relative Sanghuangporus species including S. sanghuang, S.vaninii and S. baumii, the phylogenetic analysis and molecular recognition of relative Sanghuangporus species were performed based on the internal transcribed spacer (ITS) sequence of the ribosome rDNA. NJ phylogenetic tree indicated that 23 S.sanghuang strains, 11 S. vaninii strains and 6 S. baumii strains formed three independent clades with a high bootstrap support rate, and the obvious differences among the three relative species were presented. Based on the difference in rDNA ITS sequence alignment of S.sanghuang and S.vaninii, two pairs of primers, Sv_U1/Sv_L and Sv_U2/Sv_L, were designed, which can specifically amplify two ITS fragments of 478 bp and 651 bp only from S.vaninii. but not from S. sanghuang, indicating that this method can be effectively used for the molecular recognition of the two relative Sanghuangporus species. This study provides the evidence for the phylogenetic relationship of Sanghuangporus genus, and an effective molecular assisted tool for the precise recognition of relative Sanghuangporus species.
