A UPLC method has been developed in the current investigation for simultaneous determination of four main iridoid glycosides, which were verproside, catalposide, amphicoside and 6-O-veratroylcatalposide in Veronica ciliata Fisch. The separation was performed at 40 ℃ on Waters ACOUITY T3 column (100 mm×2.1 mm, 1.8 μm); the mobile phase was acetonitrile-water(0.1% formic acid+2.5 mmol ammoniumformate); the flow rate was 0.4 mL/min and the detection wavelength was 260 nm. The results showed that the calibration curves were the good linear relationships in the range of 1.562～50 μg/mL (R2＝0.9987) for verproside, 1.562～50 μg/mL (R2＝0.9989) for catalposide, 4.689～143 μg/mL (R2＝0.9996) for amphicoside, 1.562～50 μg/mL (R2＝0.9998) for 6-O-veratroylcatalposide, the average recovery rate were 100.21%(RSD=1.3%), 99.43% (RSD=1.8%), 100.6% (RSD=2.5%), 102.7% (RSD=0.65%),respectively. The method is simple, sensitive, economical and reliable, and can be used for quality control and evaluation of Veronica ciliata Fisch.
