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论文摘要

不同羊肚菌干品挥发性成分检测及其差异性分析

Detection and analysis of volatile components in dried morel

作者:张福生(四川大学生命科学学院 教育部生物资源与生态环境重点实验室);龙力(四川大学生命科学学院 教育部生物资源与生态环境重点实验室);余晓瑞(四川大学生命科学学院 教育部生物资源与生态环境重点实验室);方磊(四川大学生命科学学院 教育部生物资源与生态环境重点实验室);胡宗瑞(四川大学生命科学学院 教育部生物资源与生态环境重点实验室);张予馨(四川大学生命科学学院 教育部生物资源与生态环境重点实验室);喻东(四川大学生命科学学院 教育部生物资源与生态环境重点实验室);龙章富(四川大学生命科学学院 教育部生物资源与生态环境重点实验室)

Author:ZHANG Fu-Sheng(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University);LONG Li(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University);YU Xiao-Rui(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University);FANG Lei(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University);HU Zong-Yue(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University);ZHANG Yu-Xin(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University);YU Dong(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University);LONG Zhang-Fu(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)

收稿日期:2019-02-20          年卷(期)页码:2019,56(5):963-970

期刊名称:四川大学学报: 自然科学版

Journal Name:Journal of Sichuan University (Natural Science Edition)

关键字:羊肚菌;顶空固相微萃取;气相色谱-质谱联用分析;挥发性香气成分;主成分分析

Key words:Morel; HS+SPME; GC-MS; Volatile components; PCA

基金项目:四川省科技培训项目(2016KZ0006)、凉山州产学研合作项目(17CXY0015

中文摘要

为探究不同品种羊肚菌干品挥发性成分及其差异性,采用顶空固相微萃取(HS-SPME)联用气相色谱-质谱(GC-MS)分析的方法对 M2、M3、M4、M5和M6 共5个人工栽培品种羊肚菌干品挥发性成分进行检测和分析.从 5 个品种中共检测出173种化合物,其中不同化合物134种,分别为 31 种烷烃、19 种醇类、16种醛类、12种胺类、9种酸类、8种烯烃、8种酯类、6种酮类、4种苯类、4种吡嗪、3种炔烃、3种呋喃、12种其它类型化合物; 在2个及以上品种中含有的非特征化合物共46种,在各品种中的相对含量为70.13%~90.15%;只在1个品种中检测到的特征化合物共88种,在各品种中的相对含量为9.85%~29.87%. 主成分分析(PCA)表明不同品种间挥发性成分的差异大小与品种间亲缘远近关系表现出相一致的特点;检测结果表明特征化合物种类少、但含量高,是羊肚菌挥发性成分的主体,而非特征化合物种类多、含量却较低是不同品种间香气成分差异的原因.

英文摘要

In order to explore the volatile components and their differences among five varieties of dried morels, the methods of headspace solid+phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) was used to analyze the volatile components in varieties of M2, M3, M4, M5, and M6. The results showed that a total of 173 compounds were detected from 5 varieties, of which 134 were different compounds, including 31 alkanes, 19 alcohols, 16 aldehydes, 12 amines, 9 acids, 8 olefins, 8 esters, 6 ketones, 4 benzenes, 4 pyrazines, 3 alkynes, 3 furans, and 12 others. There are 46 kinds of non characteristic compounds in 2 or more varieties, and the relative content in each variety was 70.13%~90.15%. A total of 88 kinds of characteristic compounds were detected in only 1 variety and the relative contents of which was 9.85%~29.87%. The PCA analysis indicated that the difference in the size of volatile components among varieties showed consistent characteristics with the close relationship among them. The results showed that the characteristic compounds are few kinds but higher contents, which is the main volatile components of the morel. The non characteristic compounds contents are low but more species, and it is the reason for the difference of aroma components among different varieties.

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