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论文摘要

甘薯贮藏期间淀粉酶种类变化及其部分性质分析

Analysis of the part charateristics and the difference of amylase in tuberous roots of sweet potato during storage period

作者:张勇为(四川大学生命科学学院, 四川省分子生物学与生物技术重点实验室);张义正(四川大学生命科学学院, 四川省分子生物学与生物技术重点实验室);谭文芳(四川省农业科学院作物研究所);杨松涛(四川省农业科学院作物研究所)

Author:ZHANG Yong-Wei(Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University);ZHANG Yi-Zheng(Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University);TAN Wen-Fang(Crops Research Institute, Sichuan Academy of Agricultural Science);YANG Song-Tao(Crops Research Institute, Sichuan Academy of Agricultural Science)

收稿日期:2017-02-11          年卷(期)页码:2018,55(1):0197-0200

期刊名称:四川大学学报: 自然科学版

Journal Name:Journal of Sichuan University (Natural Science Edition)

关键字:甘薯 块根 淀粉酶 敏感性

Key words:sweet potato(Ipomoea batatas Lam),tuberous root,amylase, sensitivity

基金项目:省自然科学基金

中文摘要

随着人们生活水平的提高和营养保健意识的增强, 甘薯在营养平衡及健康等方面的意义越来越受到人们的重视。相对于其它粮食作物,甘薯的许多基本生理活动研究相对较少。本文就甘薯收获后,自然储藏期间,基本生理活动之一的淀粉酶,其种类变化和部分性质进行研究。从收获开始,每间隔2周取样1次,提取可溶性总蛋白,通过连续聚丙烯酰胺凝胶电泳(PAGE)及淀粉酶活性染色,结果显示:在贮藏期间,有新的淀粉酶活性带出现,且新出现的淀粉酶活性带,在性质上与原有淀粉酶活性带存在差异。主要表现在,对β-巯基乙醇和EDTA均非常敏感,而原来的淀粉酶活性带几乎不敏感;在热稳定性方面新出现的淀粉酶也更为敏感。

英文摘要

As compared with other crops, the researches of sweet potato has long-term not been enough concerned, resulting in slow progress in molecular biology accumulation. Differences amylase were found in isozyme composition in tuberous roots of sweet potato during storage period, by eletrophoresis on 8% native polyacrylamide gel and active stain by using soluble starch as substrate. New amylase responds differently to inhibitors, it is sensitive to EDTA or thiol -group reagent, it is more sensitive to tompurature than β-amylase .

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