The pot-experiment was conducted to analysis the absorption characteristics of oilseed rape and the change of soil microbial community structure under different Cd concentrations. Results indicated that the absorption of Cd in the plant increased significantly as the rising of Cd concentrations, and the content in the shoot was higher than that of roots. Cd concentration in stem was the highest, and Cd concentration in seed was the lowest. The translocation factors (TFs) of Cd in oilseed rape showed a trend of first increasing and then decreased with the rising of Cd concentrations. At a low Cd concentration, the translocation factors (TFs) of Cd in oilseed rape was higher and the remediation rate can reach 6.841~7.752%. Soil microbial diversity analysis showed that Cd contamination altered soil microbial community structure and reduced the relative abundance of some dominant bacteria, but cultivation of oilseed rape can increase the relative abundance of them, and increased the soil microbial diversity. Therefore, cultivation of oilseed rape is beneficial to the stability of soil ecosystem and can be used to remediate the Cd-contaminated soil.