In order to establish a rapid and accurate determination method for measurement of total flavonoids in bamboo leaves, a general colorimetric method was optimized by using the alkaline sample solution as the reference solution, using isoquertoside as the standard, and shortening the staining time after adding the staining reagent. The optimized colorimetric method reduced the measurement time by 1/3 while reducing the impurities interference and eliminating the measurement error caused by the rutin standard. Furthermore, the method provided good linearity and precision. The average recovery rate was 100.14%, and the RSD was 0.79%(n=5). The results showed that the optimized colorimetric method can be used for the determination of total flavonoids in bamboo leaves.
