Based on previous obtained transcriptome data of the larval gut of Apis cerana cerana, a total of 57327 alternative splicing events occurred in 9124 genes were identified, in normal guts (AcCK) and Ascosphaera apis infected guts (AcT1, AcT2 and AcT3). among them, intergenic(17.68%), p3 splice (15.32%), exon span (ES) (14.12%) and p5 splice (12.81%) were main kinds of alternative splicing. Venn analysis showed 8111 alternatively spliced genes were shared in these four samples, and the number of unique alternatively spliced genes in AcT1, AcT2 and AcT3 are 272, 189 and 385, respectively. GO categorization demonstrated the shared alternative spliced genes were engaged in 47 GO terms, and the unique alternatively spliced genes in AcT1, AcT2 and AcT3 were related to 24, 20 and 34 GO terms. KEGG pathway enrichment analysis displayed the shared alternatively spliced genes were enriched in 327 pathways, and the largest ones were RNA transport, protein processing in endoplasmic reticulum and ribosome; the unique alternatively spliced genes of AcT1, AcT2 and AcT3 were enriched in 22, 46 and 83 pathways, respectively.
