The absorption characteristics and physiological responses of oilseed rape transformed by PprI were analyzed by the outdoor pot experiment under the stress of different concentrations of cadmium. Results indicated that the accumulation of Cd in both transgenic and wild type plants all increased significantly as the increase of Cd concentration in soil, and the content in roots was higher than upon-ground part. Under the 0.5 mg/kg Cd in soil, there were no significant differences between the accumulations of Cd in the two genotype oilseed rapes, but the accumulation of Cd in the transgenic plants was significantly lower than that in wild type at 1 and 2 mg/kg Cd treatments. The accumulation coefficient and translocation coefficient of plant reduced gradually with the increasing concentration of Cd. Under the stress of cadmium, superoxide dismutase (SOD) activity in all plants showed a trend of first increasing and then decreasing and SOD activity in all transgenic plants were higher than that in wild type. The content of malondialdehyde was increased, and the content of transgenic rapeseed was lower than that of non transgenic wild rape. The content of chlorophyll a and chlorophyll b in all plants decreased with the increase of Cd concentration. Therefore, the introduction of PprI gene significantly reduced the absorption of Cd in rapeseed, and increased the safety of the edible part.