Abstract：Objective The blood products packaging materials of rubber closures in the process of sample store, there might be 2,6-di-tert-butylphenol dissolution, so it is need to establish the 2,6-di-tert-butylphenol testing method of blood products. Method According to the properties of intravenous human immunoglobulin (pH4) and human serum albumin, perchloric acid was selected as precipitant reagent and tributyl phosphate as internal standard to detect the 2,6-di-tert-butylphenol content .Then the method is verified by the specificity, accuracy, repeatability, linearity, limit of quantification, range and intermediate precision. Result The results demonstrated this method was feasible without protein interference, 2,6-di-tert-butylphenol and the internal standard of tributyl phosphate were well separated in this methold. Moreover, each verification result was consistent with the expectation, and the limit of quantitation was 0.3 ng/mL. Therefore, the testing method was proved to be very effective. Conclusion In this work, a simple and sensitive method was first developed and successfully applied to detect the content of 2,6-di-tert-butylphenol from blood products.
