HPLC测定香椿中的有机酸和黄酮等7种活性成分
Determination of Six Flavonoids in Agrimonia Pilosa Ledeb by HPLC
作者：高意(西南大学化学化工学院发光与实时分析教育部重点实验室)；周光明(西南大学化学化工学院发光与实时分析教育部重点实验室)；张彩虹(西南大学化学化工学院发光与实时分析教育部重点实验室)；于璐(西南大学化学化工学院发光与实时分析教育部重点实验室)；陈军华(西南大学化学化工学院发光与实时分析教育部重点实验室)；廖安辉(西南大学化学化工学院发光与实时分析教育部重点实验室)
Author：GAO Yi(Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of CHemistry and Chemical Engineering, Southwest University)；ZHOU Guang-Ming(Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of CHemistry and Chemical Engineering, Southwest University)；ZHANG Cai-Hong(Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of CHemistry and Chemical Engineering, Southwest University)；YU Lu(Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of CHemistry and Chemical Engineering, Southwest University)；CHEN Jun-Hua(Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of CHemistry and Chemical Engineering, Southwest University)；LIAO An-Hui(Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of CHemistry and Chemical Engineering, Southwest University)
收稿日期：2016-03-23 年卷（期）页码：2017,54(5):1039-1044
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：高效液相色谱；香椿；超声萃取；黄酮；有机酸；含量测定
Key words：HPLC; Toona sinensis; ultrasonic extraction; Flavonoids; organic acid; content determinatio
基金项目：国家自然科学基金面上项目21277110
中文摘要
摘要：目的 建立超声辅助萃取-高效液相色谱法分离香椿中表儿茶素、没食子酸、儿茶素、芦丁、杨梅素、槲皮素和山奈酚7种活性成分及其含量测定的方法。方法 采用Phenomenex C18色谱柱 (150 mm×4.6 mm, 5 μm)分离7种成分；流动相为甲醇-0.1%醋酸溶液，梯度洗脱；流速：0.8 mL﹒min-1，紫外检测波长：290 nm，柱温：35 ℃。结果 7种成分在15 min内均达到基线分离，线性关系良好（r>0.9991, n=7），平均回收率均在99.23%～104.5% (RSD%
英文摘要
Abstract： Objective: The HPLC method is established for separation and determination of epicatechin, gallic acid, catechin, rutin, myricetin, quercetin and kaempferol in Toona sinensis by ultrasonic extraction. Methods: The separation of seven active ingredients is performed on Phenomenex C18 column (150 mm×4.6 mm, 5 μm) with step gradient. Mobile phase is methanol -0.1% acetic acid at the flow rate of 0.8 mL﹒min-1 ; the UV detection wavelength is 290 nm and the coclumn temperature is set at 35℃. Results: The good separation of epicatechin, gallic acid, catechin, rutin, myricetin, quercetin and kaempferol is achieved within 15 min. Calibration curves of the seven active ingrediens show linear relationship (r>0.9991, n=7). The average recoveries are within 99.23%～104.5% (RSD%
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