The extraction of volatile oil was performed by water vapor distillation, ultrasonic and Soxhlet extraction from saffron bio-residue. 49 kinds of compounds were identified in total and the relative content in percentage were calculated with area normalization by GC-MS. Three methods can extract 43, 27 and 21 kinds of components respectively and share 6 components together. The main components are palmitic acid, linoleic acid, linolenic acid. In contrast, water vapor distillation can harvest showed the most variety of components such as linalol，eucarvone and cedrol etc.. What’s more, the relative content of safranal was 40.31%. Soxhlet extraction can show the highest extraction rate. In the experiment of antioxident activity, three extracts were tested by DPPH, reducing power and T-AOC for antioxidant measurement, respectively. The clearance rate of water vapor distillation extracts reached 44.17%, which was the highest in the three extraction methods when the steam distillation’s extract concentration at 280μg/ml. The extract by Soxhlet extraction showed the best result in reducing power and T-AOC tests.
