A full length GDP D mannose pyrophosphorylase (GMP) cDNA from Pogonatherum paniceum was cloned through RACE method using known GMP gene fragments and named PH GMPase (GeneBank Accession Number: KF586841). The PH GMPase ORF contains 361 amino acids and the molecular formula of it is C1787H2874N474O509S17. It′s DNA Sequence is consist of 4 exons and 3 introns. The sequence homology analysis shows PH GMPase has a 98.06%, 93.07%, 88.64%, 86.98%, 87.53% and 86.43% similarity with Zea mays, Oryza Sativa, Actinidia chinensis, Nicotiana tabacum, Arabidopsis thaliana and Manihot esculenta, respectively. The constructed phylogenetic tree indicates PH GMPase has a common ancestor with other higher plants, fungi, higher animals and bacteria and has a nearest relationship with Brachypo diumdistachyon. The expression level of GMP was determined under salt, drought and cold stress by fluorescent quantization. The result showed GMP was up regulated in both three stress conditions, and the content of ascorbic acid (AsA) subsequently increased.
