AtMYB44与ABI1竞争性结合ABA受体RCAR1的研究

The research of AtMYB44 and ABI1 via competitive binding to ABA receptor RCAR1 in Arabidopsis thaliana

作者：赵哲(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；李德款(四川大学生命科学学院)；袁德志(四川大学生命科学学院)；王晓玉(四川大学生命科学学院)；李旭锋(四川大学生命科学学院生物资源与生态环境教育部重点实验室)

Author：ZHAO Zhe(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；LI De-Kuan(College of Life Sciences, Sichuan University)；YUAN De-Zhi(College of Life Sciences, Sichuan University)；WANG Xiao-Yu(College of Life Sciences, Sichuan University)；LI Xu-Feng(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)

收稿日期：2013-12-26 年卷（期）页码：2015,52(3):663-667

期刊名称：四川大学学报: 自然科学版

Journal Name：Journal of Sichuan University (Natural Science Edition)

关键字：竞争性蛋白沉降技术; RCAR1; ABI1; AtMYB44

Key words：Competitive pull down; RCAR1; ABI1; AtMYB44

基金项目：国家自然科学基金（31171586）; 国家转基因重大专项（2011ZX08009 003 002）

中文摘要

为了进一步确定AtMYB44、ABI1、RCAR1三者之间的关系，本文进行了竞争性pull down和ABI1磷酸酶活性的测定实验，结果表明AtMYB44与ABI1能够竞争性结合RCAR1，并且这种竞争性结合依赖于ABA，AtMYB44能够降低RCAR1对ABI1活性的抑制，这为深入研究ABA信号转导途径奠定了基础.

英文摘要

In order to determine the relationship among the AtMYB44,ABI1 and RCAR1, competitive GST pull down assay and ABI1 phosphatase activity assay were used in this study, these results suggested that AtMYB44 and ABI1 compete in vitro for binding to RCAR1 in the presence of ABA. In addition, AtMYB44 reduces the inhibitory effect of RCAR1 on ABI1 phosphatase activity in the presence of ABA, which build the foundation for the ABA signal pathway study.

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论文摘要

AtMYB44与ABI1竞争性结合ABA受体RCAR1的研究

The research of AtMYB44 and ABI1 via competitive binding to ABA receptor RCAR1 in Arabidopsis thaliana