煤基活性炭共价固载葡萄糖氧化酶及其在生物传感器中的直接电化学性能
Glucose oxidase covalent immobilized on coal based activated carbon and direct electrochemical performance in biosensor
作者：肖宇(海南大学材料与化工学院海南省玻璃重点实验室)；丁春华(海南大学材料与化工学院海南省玻璃重点实验室)；汪国庆(海南大学材料与化工学院海南省玻璃重点实验室)；姜宏(海南大学材料与化工学院海南省玻璃重点实验室)；林仕伟(海南大学材料与化工学院海南省玻璃重点实验室)
Author：XIAO Yu(College of Material and Chemistry, Hainan Key Lab of Glass, Hainan University)；DING Chun-Hua(College of Material and Chemistry, Hainan Key Lab of Glass, Hainan University)；WANG Guo-Qing(College of Material and Chemistry, Hainan Key Lab of Glass, Hainan University)；JIANG Hong(College of Material and Chemistry, Hainan Key Lab of Glass, Hainan University)；LIN Shi-Wei(College of Material and Chemistry, Hainan Key Lab of Glass, Hainan University)
收稿日期：2013-12-19 年卷（期）页码：2015,52(3):625-630
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：棒状煤基活性炭; 固定化; 葡萄糖氧化酶; 循环伏安法
Key words：Cylindrical coal based activated carbon; Immobilization; GOD; Cyclic voltammetry
基金项目：海南大学青年基金项目（qnjj1233）
中文摘要
在棒状煤基活性炭上进行氧化、硅烷偶联剂(APTMS)的硅烷化, 并与戊二醛交联, 成功共价固载了葡萄糖氧化酶(GOD). 葡萄糖氧化酶固载量为74.75 mgGOD/g载体, 固载酶的最佳活性pH为6.0, 最佳活性温度为38 ℃. 本实验第一次以固载GOD棒状煤基活性炭作为工作电极, 铂丝为辅助电极, 饱和甘汞电极(SCE)作为参比电极测得循环伏安曲线, 得到一对氧化还原峰, 随着扫速的增加电位差增大, 表明GOD ACAGM电极能够对该酶促反应有很好的响应.
英文摘要
GOD(glucose oxidase) was successfully immobilized onto cylindrical coal based activated carbon through oxidation, aminosilylation and aldehyde grafting modification.The amount of immobility on cylindrical coal based activated carbon was 74.75 mg/g,and the best activity of immobilized enzyme at a temperature of 38 ℃ and pH 6.This was the first experiment about cylindrical coal based activated carbon as working electrode,Pt as the counter electrode, saturated calomel electrode as the reference electrode. Cyclic voltammetry showed that we got a pair of redox peak,and with increasing scan rate,the peak separation begins to increase, it showed that the electrode of GOD ACAGM(the electrode which Covalent immobilized GOD) had good response to enzyme catalysis.
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