微生物电解乙酸合成高值有机物
Study of high value organics synthesis from acetate reduction in microbial electrochemical systems
作者：王娟(四川大学生命科学学院)；朱晓宇(中国科学院成都生物研究所)；刘柯(中国科学院成都生物研究所)；李建(四川大学生命科学学院)；李大平(中国科学院成都生物研究所)；高平(四川大学生命科学学院)
Author：WANG Juan(College of Life Sciences, Sichuan University)；ZHU Xiao-Yu(Chengdu Institute of Biology, Chinese Academy of Science)；LIU Ke(Chengdu Institute of Biology, Chinese Academy of Science)；LI Jian(College of Life Sciences, Sichuan University)；LI Da-Ping(Chengdu Institute of Biology, Chinese Academy of Science)；GAO Ping(College of Life Sciences, Sichuan University)
收稿日期：2014-04-01 年卷（期）页码：2015,52(4):925-931
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：微生物电化学技术; 生物阴极; 乙酸; 丁酸; 乙醇
Key words：Microbial electrochemical technology; Biocathode; Acetate; Butyrate; Ethanol
基金项目：国家自然科学基金资助项目(31270166)
中文摘要
以微生物电解池为平台, 考察了阴极电势, pH以及初始乙酸盐浓度对乙酸还原及其丁酸、乙醇等产物积累的影响.结果显示, 恒定阴极电势-900mV(vs.Ag/AgCl)、 pH60时, 初始乙酸钠浓度为10g/L时, 丁酸产量最高, 可达到80(±5)mg/L, 乙醇积累26(±5)mg/L.在-900mV阴极电势下, 总碳回收率达到632%, 产物电子回收率为1306%; 在-800mV阴极电势下, 总碳回收率达到428%, 产物电子回收率为544%.控制阴极电势-850mV, 初始乙酸钠浓度由1g/L增加到5g/L, 丁酸的产量由488mg/L增加到762mg/L, 提高了56%.扫描电镜显示, 阴极碳毡上菌体主要为杆菌.对阴极附着微生物进行变性梯度凝胶电泳(PCR DGGE)分析发现, 假单胞菌属(Pseudomonas)和梭菌属(Clostridium)为主要的功能菌群, 假单胞菌可能与阴极电子传递有关, 而梭菌可能与高值有机物的合成有关.
英文摘要
The effect of cathode, pH and initial concentration of acetate on the acetate reduction and the accumulation of butyrate, ethanol and other products were studied taking microbial electrochemical technology as a platform. In the batch test of biocathode potential, the highest production rate of butyrate and ethanol was 80 ( ± 5) mg/L and 26 ( ± 5) mg/L (at potential-900 mV). Electron and carbon balances revealed that the cathodic carbon efficiency and electron efficiency was 632% and 1306% at potential of -900 mV. At potential of -800 mV, the cathodic carbon efficiency and electron efficiency was 428% and 544%, respectively. Batch test of pH indicated that pH60 was the best condition of producing high value organics. At the potential of -850 mV, the yield of butyrate increased from 488mg/L to 762mg/L, the concentration of butyrate increased by 56% from 1 g/L to 5 g/L sodium acetate. SEM showed the cathodes mainly included rod shaped organism. PCR DGGE revealed that Pseudomonas and Clostridium were dominant populations. Pseudomonas may be associated with the cathode electron transfer, and clostridium may be associated with the synthesis of high value organics.
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