黄瓜幼苗对外源氰化物耐受性研究

Putative mechanism of cucumber seedlings tolerance to exogenous cyanide

作者：吴阳晨(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；徐飞(武汉生物工程学院生物科学和生物技术系)；林宏辉(四川大学生命科学学院生物资源与生态环境教育部重点实验室)

Author：WU Yang-Cheng(key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；XU Fei(Life Science and Biotechnology, Wuhan Bioengineering Institute)；LIN Hong-Hui(key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)

收稿日期：2014-03-31 年卷（期）页码：2015,52(4):917-924

期刊名称：四川大学学报: 自然科学版

Journal Name：Journal of Sichuan University (Natural Science Edition)

关键字：交替氧化酶; 氰化物; β 氰丙氨酸合成酶; 黄瓜

Key words：Alternative oxidase; Cyanide; β cyanoalanine synthase; Cucumber

基金项目：国家自然科学基金（91417305，1470342，1400242）；四川省科技厅项目（2015JY0101，2015JY0223）

中文摘要

用不同浓度的氰化钾（KCN）溶液处理黄瓜幼苗，发现黄瓜幼苗可以耐受较高浓度（1mM）外源氰化钾胁迫的能力.氰化钾预处理明显诱导交替氧化途径并激活β 氰丙氨酸合成酶（β CAS）.研究结果表明，交替氧化途径和β CAS对减轻呼吸抑制是必不可少的，可以提高植物对外源氰化物的耐受力并维持正常生长状态.

英文摘要

In this study, different concentration of potassium cyanide (KCN) had been used to treat cucumber seedlings and found that it had a good tolerance to higher concentration (1mM) of exogenous cyanide treatment. Physiological analyses showed that cyanide pretreatment significantly induced the alternative oxidase (AOX) pathway and activated the β cyanoalanine synthase (β CAS). The results indicated that the AOX pathway and β CAS were essential to alleviate respiration inhibition and then contributed to normal growth during plant response to exogenous cyanide.

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论文摘要

黄瓜幼苗对外源氰化物耐受性研究

Putative mechanism of cucumber seedlings tolerance to exogenous cyanide