藏红花腐烂病病原真菌的分离鉴定及药剂防治
Identification and fungicides screening of Saffrons rot disease pathogen
作者：周双(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；白洁(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；陈放(四川大学生命科学学院, 能源植物生物燃油制备及利用国家地方联合工程实验室)
Author：ZHOU Shuang(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；BAI Jie(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；CHEN Fang(National Local Joint Engineering Laboratory for Energy Plant Bio oil Production and Application, College of Life Sciences, Sichuan University)
收稿日期：2014-03-25 年卷（期）页码：2015,52(4):911-916
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：藏红花; 腐烂病; 核糖体DNA转录间隔区; 抑菌药剂
Key words：Saffron; rot disease; rDNA ITS; fungicides screening
基金项目：西红花规模化生产技术服务（HS20100601105584）
中文摘要
为明确藏红花腐烂病的病原真菌种类, 对感病藏红花球茎进行发病组织培养、病原菌分离与单孢纯化. 将获得的菌株进行病原菌致病性检测验证后, 结合真菌形态学及rDNA ITS序列BLAST比较分析, 发现尖孢镰刀菌株是藏红花腐烂病的主要致病菌. 采用常用抑菌菌剂对病原菌进行抑菌测定, 结果表明:75%代森锰锌可湿性粉剂等4种抑菌剂对病原菌抑菌率达60%以上.
英文摘要
In order to ascertain the fungi which cause rot of Saffrons corm, fungi were isolated from a large number of rotten corms on PDA medium, and purified by single spore culture, respectively. The results revealed that Fusarium oxysporum f. sp. ciceris was the main pathogens of Saffrons corm rot disease according to the rDNA ITS sequence and the characteristics of fungal morphology. Then screen out effective chemical for inhibition of its mycelium growth. Mycelium growth could be controlled effectively by mancozeb 75% WP etc.
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