低表达长非编码RNA MALAT1对人宫颈癌HeLa细胞体外生长和迁移的影响
Lower expression lncRNA MALAT1 effect on the proliferation and migration of HeLa cells in vitro
作者：齐文川(四川大学生命科学学院功能基因组实验室)；李骞(四川大学生命科学学院功能基因组实验室)；吴传芳(四川大学生命科学学院功能基因组实验室)
Author：QI Wen-Chuan(Department of Functional Genome, College of Life Sciences, Sichuan University,)；LI Qian(Department of Functional Genome, College of Life Sciences, Sichuan University,)；WU Chuan-Fang(Department of Functional Genome, College of Life Sciences, Sichuan University,)
收稿日期：2014-03-24 年卷（期）页码：2015,52(4):901-905
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：HeLa细胞; 长非编码RNA MALAT1; 增殖与迁移; siRNA与shRNA干扰
Key words：HeLa Cells; lncRNA MALAT1; Proliferation and migration; siRNA and shRNA interference
基金项目：国家自然科学基金面上项目（31371325）
中文摘要
设计3个针对MALAT1不同靶序列的小干扰RNA(small interfering RNA, siRNA), 筛选出有效的siRNA序列, 设计并构建短发夹RNA(short hairpin RNA, shRNA)干扰质粒, 转染到HeLa细胞中, 构建低表达MALAT1的稳定细胞株.通过细胞的生长曲线和细胞划痕实验验证降低MALAT1对HeLa细胞增殖和迁移的影响.结果显示MALAT1表达水平在HeLa细胞中得到有效地降低, 低表达MALAT1的HeLa细胞生长速度和迁移速度减小.表明MALAT1在HeLa细胞中具有调控细胞增殖和迁移的能力
英文摘要
Three small interfering RNAs (siRNAs) which target different sequences of MALAT1 were designed and picked out the most effective one. Based on this siRNA, the short hairpin RNA(shRNA)plasmids were constructed, which was stably transfected into HeLa cells, and lowered down the expression of MALAT1. Through the cell growth curve and wound scratch assay, down regulation of MALAT1 may affect proliferation and migration of HeLa cells were tested. Results showed that the expression level of MALAT1 in HeLa cells could be effectively lowered, low expression of MALAT1 reduced the proliferation and migration. In HeLa cells, MALAT1 has the ability to regulate cell proliferation and migration.
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