番茄MSI2 like基因的克隆及功能初探
Cloning of MSI2 like and initial exploration of gene function in tomato
作者：李頔(四川大学生命科学学院水力学与山区河流开发保护国家重点实验室/生物资源与生态环境教育部重点实验室)；唐晓凤(合肥工业大学生物与食品工程学院)；刘永胜(四川大学生命科学学院水力学与山区河流开发保护国家重点实验室/生物资源与生态环境教育部重点实验室)
Author：LI Di(State Key Laboratory of Hydraulics and Mountain River Engineering / Key Laboratory of Bio resource and Eco environment of Ministry of Education, College of Life Sciences, Sichuan University)；TANG Xiao-Feng(School of Biotechnology and Food Engineering, Hefei University of Technology)；LIU Yong-Sheng(State Key Laboratory of Hydraulics and Mountain River Engineering / Key Laboratory of Bio resource and Eco environment of Ministry of Education, College of Life Sciences, Sichuan University)
收稿日期：2014-03-02 年卷（期）页码：2015,52(4):871-878
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：番茄; MSI2 like基因; 酵母双杂交; RNA干涉; 干旱胁迫
Key words：Tomato; MSI2 like gene; Yeast two hybrid analysis; RNA interference; Drought stress
基金项目：教育部博士点基金项目(20110181130009)； 国家杰出青年科学基金项目(30825030)； 国家自然科学基金项目 (31171179).
中文摘要
研究通过对番茄MSI2 like蛋白序列与其它模式植物的蛋白序列进行同源性比对, 发现番茄MSI2 like与马铃薯的同源性最高; 另外还预测了其蛋白质的理化性质及三级结构. 酵母双杂交实验证明, MSI2 like与UV Damaged DNA Binding Protein 1(DDB1)具有相互作用. 同时, 利用RNA干涉技术构建植物双元载体, 农杆菌介导法转化番茄得到转基因植株, 该转基因植株在干旱胁迫条件下有一定的抗旱功能. 再利用实时荧光定量PCR分析MSI2 like基因表达的组织差异性, 发现它在各个组织中都表达, 但是在生殖器官花中的表达量明显较其他组织高.
英文摘要
Tomato MSI2 like has the highest homology with potato through sequence homology analysis; in addition, physical and chemical properties and protein tertiary structure were predicted. The results of yeast two hybrid analysis showed that tomato MSI2 like interacts with UV Damaged DNA Binding Protein 1 (DDB1). Meanwhile, RNA interference binary vector was constructed and introduced into Solanum lycopersicum L. cv. Ailsa Craig by means of Agrobacterium mediated transformation; transgenic lines enhanced the germination of transgenic seeds tolerance to drought to some extent. Real time PCR analysis indicated that MSI2 like gene expressed in all tested tissues, but the expression level of MSI2 like was much higher in reproductive organs, namely flower, than in other tissues.
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