家猪STAT1的基因克隆、剪接变体鉴定及组织表达研究

Signal transducer and activator of transcription 1 gene (STAT1) in pig: cDNA cloning, tissue distribution, and identification of the alternative splicing variants

作者：付浩(四川大学生命科学学院功能基因组实验室)；孟凤艳(四川大学生命科学学院功能基因组实验室)；李芳莲(四川大学生命科学学院功能基因组实验室)；李娟(四川大学生命科学学院)

Author：FU Hao(College of Life Sciences, Sichuan University)；MENG Feng Yan(College of Life Sciences, Sichuan University)；LI Fang Lian(College of Life Sciences, Sichuan University)；LI Juan(College of Life Sciences, Sichuan University)

收稿日期：2013-04-11 年卷（期）页码：2015,52(5):1157-1163

期刊名称：四川大学学报: 自然科学版

Journal Name：Journal of Sichuan University (Natural Science Edition)

关键字：家猪; STAT1; 剪接变体; 组织表达

Key words：Pig; STAT1; Splice variants; Tissue expression

基金项目：国家重大专项转基因生物新品种培育 (2009ZX08009-145B)

中文摘要

以长白猪(Landrace)cDNA为模板, 克隆STAT1基因cDNA全长.家猪STAT1基因cDNA全长编码757个氨基酸的前体蛋白, 与人、大鼠、小鼠、牛等哺乳动物的STAT1氨基酸序列一致性分别为95％、908％、896％、916％; 同时, 本研究亦鉴定到家猪STAT1的3种剪接变体(分别命名为STAT1 sv1, STAT1 sv2, 和STAT1 sv3).采用RT PCR方法, 进一步检测了STAT1及其3种剪接变体在成年家猪各组织中的表达情况.STAT1和两种剪切变体(STAT1 sv2 和STAT1 sv3)在家猪组织中广泛表达, 而剪接变体STAT1 sv1则在肺和脾脏中有较高表达水平.

英文摘要

In this study, the full length cDNAs of pig STAT1 (pSTAT1) gene was cloned using RT PCR. The cloned pSTAT1 encodes a pro protein of 757 amino acids, which shares high amino acid sequence identity with that of human (95 %), mouse (896 %), rat (908%) and cattle (916%). In addition, 3 novel mRNA splice variants of STAT1, named pSTAT1 sv1, pSTAT1 sv2 and pSTAT1 sv3 respectively, were identified in this study . RT PCR assay showed that STAT1 and 2 splice variants (pSTAT1 sv2 and pSTAT1 sv3) are expressed in all tissues examined, while pSTAT1 sv1 is abundantly expressed in spleen and Lung. These findings pave the way for further elucidation of the roles of STAT1 gene in pigs.

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论文摘要

家猪STAT1的基因克隆、剪接变体鉴定及组织表达研究

Signal transducer and activator of transcription 1 gene (STAT1) in pig: cDNA cloning, tissue distribution, and identification of the alternative splicing variants