Sch9通过Cdc34调控酵母细胞的寿命和胁迫应答
Sch9 regulates thelongevity and stress response by Cdc34 in yeast
作者：郄蓓蓓(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；闾 磊(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；吕 舟(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；杜林方(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；刘 科(四川大学生命科学学院生物资源与生态环境教育部重点实验室)
Author：QIE Bei Bei(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；LV Lei(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；LV Zhou(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；DU Lin Fang(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)；LIU Ke(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)
收稿日期：2014-05-05 年卷（期）页码：2015,52(5):1145-1150
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：Sch9; Cdc34; 磷酸化; PDK1位点; 寿命; 胁迫
Key words：Sch9; Cdc34; Phosphorylation; PDK1 site; Lifespan; Stress
基金项目：国家自然科学基金资助项目(30671181/C0603)
中文摘要
为研究Sch9激酶的生理功能，本文首先通过同步细胞周期的方法发现Sch9的PDK1位点的磷酸化随细胞周期变化.接下来在酵母中过表达Cdc34显示Cdc34的组成性表达可以进一步延长s ch9△突变体的寿命并且增强其对氧化胁迫及热胁迫的抗性.我们的研究结果首次表明Cdc34作为Sch9的底物参与细胞胁迫应答和衰老的调控.
英文摘要
With the purpose of examining the physiological function of Sch9 kinase, the variable phosphorylation statuses of PDK1 site of Sch9 during different cell phases were monitored using synchronized yeast. Additionally, Overexpressing Cdc34 in Sch9 deficient yeast cells was able to further lengthen the longevity and increased cellular resistance to oxidative and thermal stress. These data imply for the first time that Cdc34 participated in longevity regulation and stress response as a substrate of Sch9.
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