拟南芥中参与盐胁迫应答的基因
At1g14260的功能初探
Primary exploration of genes At1g14260 participate in salt stress response in Arabidopsis thaliana
作者:杨昊(四川大学生命科学学院生物资源与生态环境教育部重点实验室);杨凤玺(四川大学生命科学学院生物资源与生态环境教育部重点实验室);袁德志(四川大学生命科学学院生物资源与生态环境教育部重点实验室);刘志斌(四川大学生命科学学院生物资源与生态环境教育部重点实验室);杨毅(四川大学生命科学学院生物资源与生态环境教育部重点实验室)
Author:YANG Hao(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University);YANG Feng Xi(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University);YUAN De Zhi(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University);LIU Zhi Bin(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University);YANG Yi(Key laboratory of Bio resources and Eco environment of Ministry of Education,College of Life Sciences, Sichuan University)
收稿日期:2014-02-26 年卷(期)页码:2015,52(6):1347-1352
期刊名称:四川大学学报: 自然科学版
Journal Name:Journal of Sichuan University (Natural Science Edition)
关键字:拟南芥; 盐胁迫; 萌发率; 幼苗生长; 根长; 亚细胞定位
Key words:Arabidopsis thaliana; Salt stress; Germination rate; Seedling growth; Root length; Subcellular localization
基金项目:国家自然科学基金(31171586); 国家转基因重大专项(2011ZX08009 003 002)
中文摘要
拟南芥中未知基因At1g14260的表达受到多种非生物胁迫的诱导, 特别是在NaCl的诱导下, At1g14260的表达量明显增加. 对T DNA插入突变体at1g14260(salk 118406)的分析表明, 敲除了基因At1g14260的拟南芥相比野生型对盐胁迫更加敏感. 此外, 构建了融合表达载体PBi221 At1g14260 GFP并且成功转入拟南芥原生质体中, 在荧光显微镜下观察到融合蛋白定位于原生质体细胞核中. 因此, At1g14260可能参与了拟南芥中盐胁迫的过程.
英文摘要
The expression of Arabidopsis unknown gene At1g142600 was induced by a variety of abiotic stresses. The expression of At1g14260 was significantly increased under the treatment of NaCl. The results suggested that At1g14260 was involved in the process of salt stress response. Analysis of the T DNA mutant of at1g14260 (salk 118406) demonstrated that knocking out At1g14260 in Arabidopsis resulted in plants more sensitive to NaCl than the wild type. In addition, fusion vector PBi221 At1g14260 GFP was constructed and transfected into Arabidopsis protoplast. The observation by fluorescence microscope revealed that the fusion protein localized innucleus of the protoplasts. Therefore, At1g14260 may participate in the process of salt stress in Arabidopsis thaliana.
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