Objective This study aims to provide evidence for the discovery of double-sided cancer-inhibitory targets by analyzing the behavior of gene differential expression in oral squamous cell carcinoma(OSCC)’s cancer and adjacent tissues. The tissues used in the study are deposited at the National Center for Biotechnology Information(NCBI)-gene expression omnibus(GEO). Methods Expression data of OSCC cancer and adjacent tissues, as well as normal oral tissues, were downloaded from the NCBI-GEO database. After identifying differentially expressed genes, functional clustering and pathway analysis were performed. Results Most of the genes differentially expressed in cancer and adjacent tissues were similar in terms of normal oral tissues; most of these tissues have the same differential expression direction. The behavior of gene differential expression was related to its function. However, the WIF1 sample, which theoretically suppresses cell cycle, was actually up-regulated in cancer adjacent tissue. Conclusion The relationship between the genes’ function and their differential expression directions could provide evidence for double-sided target searching.
