茶多酚功能化的还原型氧化石墨烯对蛋白质的富集
Adsorption of proteins by tea polyphenols functionalized and reductive graphene oxide
作者：潘熠;李婷;康茂萍;张平;冯云;郝丽英;
Author：
收稿日期： 年卷（期）页码：2019,34(04):-359-364
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：氧化石墨烯(GO);茶多酚功能化的还原型氧化石墨烯(TPG);茶多酚;牛血清蛋白(BSA);富集;蛋白质;吸附效率;吸附容量;唾液;水热法;分子荧光法
Key words：
基金项目：国家自然科学基金资助项目(批准号:81372892);;
口腔疾病研究国家重点实验室自主开放课题(编号:SKLOD201601)
中文摘要
目的研究茶多酚功能化的还原型氧化石墨烯(TPG)对蛋白质的富集,并应用于对唾液中蛋白质的分离富集。方法采用水热法得到了TPG,使用分子荧光来考察TPG对牛血清白蛋白(BSA)及唾液中的蛋白质的富集效果。结果成功制备了TPG,且低浓度(0. 10 mg·mL~(-1)) TPG溶液在10 min内对模型蛋白BSA的富集效率超过90%,最高吸附容量可达3. 874 g·g~(-1); TPG对实际样本唾液中蛋白质的富集效率高达86%。结论 TPG对模型蛋白质及实际样本中的蛋白质都表现出了良好的富集能力,为蛋白质的富集提供了一种高效的新型碳质材料,在蛋白质的研究检测中具有良好的应用潜力。
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