可用于肼检测的新型近红外荧光探针的合成及初步性能研究
Synthesis and preliminary properties of a novel near-infrared fluorescent probe for hydrazine detection
作者：宋艳;黄文才;齐庆蓉;
Author：
收稿日期： 年卷（期）页码：2019,34(04):-325-331
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：近红外;荧光探针;4-溴丁酸酯;半花菁;肼检测;合成;紫外吸收;荧光发射;性能
Key words：
基金项目：重大新药创制综合平台建设项目(2013ZX09402103)
中文摘要
目的制备以4-溴丁酸酯为肼检测位点的近红外荧光探针(Hcy-N_2H_4),并对其进行初步性能研究。方法3-溴丙烷与2,3,3-三甲基-3H吲哚反应生成季铵盐(Cye-m),进而与2-氯-3-羟次甲基环己烯醛缩合成双取代产物(Cye),再与间苯二酚环化成半花菁染料(Hcy),最后与活化后的4-溴丁酸成酯反应,得到目标产物Hcy-N_2H_4。测定该探针在响应肼前、后的最大吸收波长、发射波长及相应的强度变化,计算探针对肼的检测限并考察其选择性。结果成功制备了探针Hcy-N_2H_4,其化学结构经~1HNMR、~(13)CNMR、HRMS确证。探针Hcy-N_2H_4的水溶性良好,对肼响应后的最大发射波长位于近红外区的715 nm,有较大的斯托克斯位移(约125 nm)。同时,该探针与肼反应前后能够通过裸眼观察到明显的颜色变化。探针Hcy-N_2H_4对肼的检测限为78. 04μmol·L~(-1),且对干扰物质的选择性良好。结论近红外荧光探针Hcy-N_2H_4具有水溶性好、检测限低、选择性高等优点,有望用于生物体内、外肼的检测。
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