桃叶珊瑚苷对脂多糖诱导的血管内皮细胞炎症损伤的保护作用
Protective effect of aucubin against lipopolysaccharide-induced human umbilical vein endothelial cells injury
作者：沈晓飞;王祎;
Author：
收稿日期： 年卷（期）页码：2019,34(05):-458-462
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：天然产物;脂多糖;桃叶珊瑚苷;内皮细胞;细胞损伤;炎症反应;丝裂原活化蛋白激酶信号通路
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基金项目：
中文摘要
目的探讨桃叶珊瑚苷对脂多糖所致血管内皮细胞损伤的保护作用。方法采用细菌脂多糖(LPS)诱导人脐静脉血管内皮细胞(HUVECs)损伤模型,经桃叶珊瑚苷(25、50、100μmol·L~(-1))处理后,以乳酸脱氢酶(LDH)漏出法检测细胞损伤情况,RT-q PCR检测促炎症因子mRNA的表达水平,Western Blotting法检测炎症相关蛋白及激酶的表达水平。结果桃叶珊瑚苷(50、100μmol·L~(-1))能明显抑制LPS所致的HUVECs细胞损伤,呈现一定的剂量依赖性。同时,桃叶珊瑚苷(50、100μmol·L~(-1))也能明显抑制白细胞介素1β、白细胞介素6mRNA的表达及LPS诱导的一氧化氮合酶、环氧化酶2蛋白的表达。此外,在50、100μmol·L~(-1)剂量下,桃叶珊瑚苷还能减弱LPS诱导的ERK1/2、p38 MAPK及JNK的磷酸化。结论桃叶珊瑚苷对LPS所致HUVECs损伤具有一定保护作用,其机制可能与抑制MAPKs炎症信号通路有关。
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