乙酰丹参酮ⅡA对SKOV3细胞增殖的抑制作用及其促进TFF1表达的作用
Inhibition the growth of SKOV3 cells and inducation the expression of TFF1 by acetyltanshione ⅡA
作者：祝飞美;李俊龙;刘蕊;黎晓龙;吴勇;刘小康;
Author：
收稿日期： 年卷（期）页码：2016,31(01):-37-40
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：丹参酮ⅡA;乙酰丹参酮ⅡA;TFF1;启动子活性;作用机制;MTT法;流式细胞术;实时荧光定量聚合酶连反应
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基金项目：
中文摘要
目的观察乙酰丹参酮ⅡA(ATA)对人卵巢癌细胞增殖和凋亡的诱导作用及对雌激素诱发蛋白TFF1表达的影响,探讨可能的作用机制。方法通过MTT法计算ATA对卵巢癌细胞的活力以及IC50值;用流式细胞术检测凋亡率及细胞周期变化情况;用实时荧光定量聚合酶连反应(RT-PCR)检测TFF1 m RNA的表达水平,并通过构建双荧光素酶报告基因系统分析ATA对TFF1启动子活性的影响。结果 ATA对多种人卵巢癌细胞系均具有增殖抑制作用,对表达雌激素受体ER的肿瘤细胞效果最明显;能使SKOV3细胞周期阻滞在G2/M期,并诱导SKOV3细胞凋亡;能促进TFF1 m RNA的表达;能增强TFF1启动子的转录活性,且在TFF1启动子区域,AP-1结合位点对TFF1启动子的活性起绝对性作用。结论 ATA能有效抑制SKOV3的生长并可促进TFF1启动子的转录活性。
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