二氢杨梅素改善抑郁样行为及应激诱导认知障碍的作用
Dihydromyricetin ameliorates depression-like behaviors and stress-induced cognitive dysfunction
作者：于旭东;曲浩天;朱晓峰;肖志勇;田绍文;
Author：
收稿日期： 年卷（期）页码：2018,33(06):-475-479
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：二氢杨梅素;认知功能障碍;自发活动;学习记忆;抑郁;应激;脑源性神经营养因子;旷场实验;Western blot法
Key words：
基金项目：
中文摘要
目的研究二氢杨梅素(DHM)对抑郁样行为及学习记忆的影响。方法将小鼠随机分成无应激+溶媒组(NS+Veh)、无应激+DHM组(NS+DHM)、应激+溶媒组(CRS+Veh)以及应激+DHM组(CRS+DHM),应激组小鼠经历10 d应激,DHM组小鼠在应激后5 d注射DHM,应激结束后所有小鼠依次进行旷场、新颖物体识别、Y迷宫、悬尾、强迫游泳等实验测试,观察DHM对小鼠的自发活动、学习记忆及抑郁样行为的作用。将WKY大鼠随机分为两组Veh组和DHM组,一部分大鼠(每组8只)依次进行旷场和FST测试,另一部分(每组4只)断头取脑,采用Western blot法检测海马内脑源性神经营养因子(BDNF)的水平。结果 CRS+Veh组小鼠在Y迷宫和新颖物体识别测试中的学习记忆能力显著低于NS+Veh组,CRS+DHM组小鼠在Y迷宫和新颖物体识别测试中的学习记忆能力显著高于CRS+Veh组,而与NS+Veh组比较,NS+DHM组在Y迷宫和新颖物体识别测试中的学习记忆能力无显著性差异; CRS+Veh组小鼠在悬尾和强迫游泳测试中的不动时间显著高于NS+Veh组,CRS+DHM组小鼠在悬尾和强迫游泳测试中的不动时间显著低于CRS+Veh组,同时,与NS+Veh组比较,NS+DHM组的悬尾和强迫游泳测试中的不动时间显著降低。在WKY大鼠中,DHM组在强迫游泳测试中的不动时间显著低于Veh组;在旷场测试中两组大鼠穿越的格子数无显著性差异。结论 DHM改善应激诱导小鼠记忆损伤,但对正常小鼠学习记忆无影响; DHM可改善正常小鼠、慢性应激小鼠、WKY大鼠的抑郁样行为以及上调WKY大鼠BDNF的表达。
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