藁本内酯对小鼠海马区损伤的保护作用及其作用机制
Protective effects of Z-ligustilide on global brain ischemia reperfusion hippocampus injury in mice and its mechanism
作者：桑纳;王平汉;杜俊蓉;
Author：
收稿日期： 年卷（期）页码：2016,31(03):-245-247
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：藁本内酯;全脑缺血再灌注;海马损伤;炎症反应;GFAP;TNFα;Nissl染色;免疫组化
Key words：
基金项目：国家自然科学基金资助项目(批准号:81072636)
中文摘要
目的研究藁本内酯(Lig)对小鼠海马区损伤的保护作用及其作用机制。方法将24只小鼠随机均分为假手术组、模型组和Lig组,采用双侧颈总动脉结扎法(2VO)建立全脑缺血再灌注模型;Nissl染色观察小鼠海马CA1、CA2区的神经元计数;免疫组化检测海马区胶质纤维酸性蛋白(GFAP)、肿瘤坏死因子(TNFα)的表达。结果与模型组比较,Lig能减轻缺血再灌带来的损伤,减少海马CA1、CA2区神经元的丢失,降低GFAP、TNFα的表达。结论 Lig能明显减轻全脑缺血再灌注所致的小鼠海马区损伤,其机制与抑制星形胶质细胞激活、减轻炎症反应有关。
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