细胞穿膜肽TAT和PEG共修饰的载阿霉素脂质体的制备及工艺的优化
The preparation and optimization of doxorubicin- loaded liposomes co- modified with cell penetrating peptide TAT and PEG
作者：曾晓芳;袁雯旻;唐洁;傅灵;何勤;
Author：
收稿日期： 年卷（期）页码：2014,29(01):-23-26
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：共修饰;TAT;PEG;脂质体
Key words：
基金项目：高等学校博士学科点专项科研基金(20090181110083)
中文摘要
目的研究细胞穿膜肽TAT和聚乙二醇(PEG)共修饰载阿霉素(DOX)脂质体的制备工艺。方法采用薄膜分散-超声法制备脂质体,主动载药法包载抗肿瘤药物DOX,以包封率、粒径为主要评价指标筛选制备工艺,并初步研究其稳定性。结果载DOX脂质体的制备工艺为:总磷脂-胆固醇(65∶35),水化溶剂为300 mmmol·L-1(NH4)2SO4缓冲液,以PBS(pH7.4)为洗脱溶剂替换外水相的硫酸铵,载药温度45℃,载药时间15 min,药脂比1∶10。所制备的载DOX脂质体性质稳定,包封率均>95%,粒径约90 nm,PDI
参考文献
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