用荧光参数表征牛血清白蛋白在脲中的变化过程
Characterize the denaturation process of bovine serum albumin in urea by fluorescence parameters
作者：张家兴;晋可;尹宗宁;
Author：
收稿日期： 年卷（期）页码：2012,27(04):-371-375
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：牛血清白蛋白;蛋白稳定性;荧光参数;色氨酸荧光;荧光探针
Key words：
基金项目：国家自然科学基金资助项目(批准号:30973659)
中文摘要
目的研究牛血清白蛋白在脲中的构象变化过程,根据荧光参数的变化,建立蛋白构象与环境变化的关系,从分子水平探讨蛋白质构象变化而致不稳定的原因。方法采用内源性荧光法、荧光淬灭法及荧光探针法研究牛血清白蛋白在脲中的构象变化过程。结果随着脲浓度的增加,牛血清白蛋白中内源性色氨酸荧光峰位先蓝移后红移,荧光强度逐步衰减;而8-苯胺基-1-萘磺酸与牛血清白蛋白结合物的荧光峰位则逐步红移,荧光强度逐渐衰减。结论在变性过程中,牛血清白蛋白的色氨酸残基所处区域构象经历了一个先紧缩后舒展的三态过程;而8-苯胺基-1-萘磺酸的探针结合位点与色氨酸残基可能位于牛血清白蛋白的不同区域,且构象变化更为灵敏。
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