流感病毒多表位基因盒的设计与预测
Design and prediction of multi-epitope gene of influenza A virus
作者：邵京京;丰锋;张强;李虹;李明远;王保宁;李婉宜;
Author：
收稿日期： 年卷（期）页码：2012,27(05):-486-489
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：流感病毒;抗原表位;生物信息学分析;多表位基因盒
Key words：
基金项目：国家自然科学基金(批准号:30973235);;
四川省科技支撑项目(批准号:2010SZ0110)
中文摘要
目的预测流感病毒抗原表位并设计合成多表位基因盒。方法收集不同类型流感病毒基因序列,联合运用Syf-peithi、ProPred、Multipred2、Bcepred、Clastal X1.83、SWISS-MODEL等多种生物信息学软件进行分析,预测流感病毒Matrix1(M1)、Matrix2(M2)、Nucleoprotein(NP)和HA蛋白上的抗原表位,设计并合成流感病毒多表位基因盒。结果在不同亚型流感病毒的HA蛋白抗原上成功筛选出2个B细胞表位,在M1、M2及NP蛋白上分别筛选出3个T细胞表位,以一定的间隔序列串联各表位,设计并合成流感多表位基因盒。预测结果显示该多表位基因盒具有良好的同源性及抗原性。结论成功设计出包含T细胞表位及B细胞表位的流感病毒多表位基因盒Eg,为流感多表位基因疫苗的研发奠定了基础。
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