人血浆中法莫替丁的测定及其药物动力学的研究
Determination of Famotidine in human plasma by SPE-LC/MS/MS
作者：桂静;杜青青;王凌;
Author：
收稿日期： 年卷（期）页码：2010,25(02):-147-150
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：法莫替丁;固相萃取;液相色谱-质谱-质谱联用法;血药浓度
Key words：
基金项目：
中文摘要
目的快速测定法莫替丁在人血浆中的浓度,并研究其药动学。方法12名健康志愿者单剂量空腹po法莫替丁片40mg,于给药后不同时间收集血浆样品,固相萃取,以阿莫西林为内标,采用液相色谱-质谱联用法测定法莫替丁在血浆中的浓度,并计算药动学参数。结果在优化条件下,法莫替丁的线性范围是0.3～400ng·mL-1(r=0.9993),最低定量限为0.3ng·mL-1,RSD=9.14%。法莫替丁片在所选受试者体内的动力学过程符合一室模型。Tmax=2.63±0.36h;Cmax=150.52±6.09ng·mL-1;AUC0-15=764.54±87.56μg·h·L-1;t1/2=2.77±0.32h。结论所建方法灵敏度高、准确度好、专属性强,分析速度快,适用于法莫替丁血药浓度的测定及其药动学的研究。
参考文献
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