贵州引种瓜蒌子乙酸乙酯提取物的α-葡萄糖苷酶抑制活性及成分分析
The α-glucosidase inhibitory activity and composition analysis of the ethyl acetate extract from introduced Trichosanthis Semen in Guizhou province
作者：梁光平;杨俊;刘亮;陈荣华;丁丽娜;万路平;陈东林;
Author：
收稿日期： 年卷（期）页码：2019,34(02):-109-114
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：瓜蒌子;黄酮;α-葡萄糖苷酶;抑制活性;负离子质谱裂解;液相色谱-质谱联用技术;成分分析;贵州省
Key words：
基金项目：贵州省科技合作计划资助项目(编号:黔科合LH字[2015]7578)
中文摘要
目的研究贵州引种瓜蒌子乙酸乙酯提取物的α-葡萄糖苷酶抑制活性及化学成分。方法以α-葡萄糖苷酶为酶源,检测不同浓度下的瓜蒌子乙酸乙酯提取物对α-葡萄糖苷酶的抑制活性。同时,采用超高效液相色谱串联三重四级杆飞行时间质谱法(UPLC-Triple-TOF/MS)进行成分检测,根据负离子质谱裂解规律以及相关文献的数据对照,分析瓜蒌子乙酸乙酯提取物的化学组成。结果在2. 0 mg·m L~(-1)浓度下,贵州引种瓜蒌子乙酸乙酯提取物对α-葡萄糖苷酶抑制活性的抑制率为88. 9%±1. 67%。同时,从引种瓜蒌子乙酸乙酯提取物中共鉴定了14种化学成分,包括7种黄酮(含1个二聚体黄酮和1个橙酮)、5种酚酸衍生物、1种单萜糖苷、1种鞣花酸,所有化合物均为首次从瓜蒌子中发现。结论瓜蒌子乙酸乙酯提取物具有α-葡萄糖苷酶抑制活性,PLC-Triple-TOF/MS方法可用于快速分析瓜蒌子乙酸乙酯提取物的化学成分,为建立贵州引种瓜蒌子快速、准确的质量评价方法提供了参考。
英文摘要
[1]中华人民共和国国家药典委员会.中国药典[S].北京:中国医药科技出版社,2015:113.
[2]田立杰.瓜蒌有效成分提取分离及对猪源链球菌抑菌活性测定[D].吉林:吉林农业大学硕士学位论文,2014.
[3]马跃平,高健,傅克玲,等.瓜蒌霜化学成分的分离与鉴定[J].沈阳药科大学学报,2010,27(11):876-879.
[4]张荣超,辛杰,郭庆梅,等.不同产区瓜蒌子有效成分含量测定[J].中国实验方剂学杂志,2013,19(18):71-73.
[5]梁光平,万路平,杨俊,等.GC-MS分析引种与原产地栝楼籽脂肪的组成[J].中国民族民间医药,2018,27(9):17-20.
[6]杨付梅,孙黔云.α-葡萄糖苷酶抑制剂微量筛选模型的正交法构建和筛选[J].中国药理学通报,2009,25(8):1113-1116.
[7]Xu M,Zhang Z,Fu G,et al.Liquid chromatography-tandem mass spectrometry analysis of protocatechuic aldehyde and its phase I and II metabolites in rat[J].J Chromatography B,2007,856(1-2):100-107.
[8]Due n~as M,Estrella I,Hernández T.Occurrence of phenolic compounds in the seed coat and the cotyledon of peas(Pisum sativum L.)[J].Eur Food Res Technol,2004,219(2):116-123.
[9]Cai WH,Matsunami K,Otsuka H,et al.Symplocosionosides AC,three megastigmane glycosides,a neolignan glucoside,and symplocosins A and B,two triterpene glycosyl esters from the leaves of Symplocos cochinchinensis var.Philippinensis[J].Amer J Plant Sci,2011,2(4):609.
[10]Hatano K,Matsui M.Syntheses of 2,4-dicyanophenoxyacetic acid and its analogues[J].Agric Biol Chem,1973,37(12):2917-2919.
[11]Pouységu L,Sylla T,Garnier T,et al.Hypervalent iodinemediated oxygenative phenol dearomatization reactions[J].Tetrahedron,2010,66(31):5908-5917.
[12]Kashiwada Y,Morita M,Nonaka G,et al.Tannins and related compounds.XCI.:Isolation and characterization of proanthocyanidins with an intramolecularly doubly-linked unit from the fern,dicranopteris pedata HOUTT[J].Chem Pharm Bulletin,1990,38(4):856-860.
[13]陈虹霞,王成章,周昊,等.漆树木粉抗氧化活性成分的HPLC-MS分析[J].林产化学与工业,2017,37(1):94-100.
[14]Gonzalez-Barrio R,Truchado P,Ito H,et al.UV and MSidentification of urolithins and nasutins,the bioavailable metabolites of ellagitannins and ellagic acid in different mammals[J].J Agric Food Chem,2011,59(4):1152-1162.
[15]Zhao Y,Wang L,Bao Y,et al.A sensitive method for the detection and quantification of ginkgo flavonols from plasma[J].Rapid Commun Mass Spectrom,2007,21(6):971-981.
[16]Nielen MWF,Van Bennekom EO,Heskamp HH,et al.Bioassay-directed identification of estrogen residues in urine by liquid chromatography electrospray quadrupole time-offlight mass spectrometry[J].Ana Chem,2004,76(22):6600-6608.
[17]Oyama K,Kondo T.Total synthesis of flavocommelin,a component of the blue supramolecular pigment from commelina communis,on the basis of direct 6-C-glycosylation of flavan[J].J Org Chem,2004,69(16):5240-5246.
[18]Ren G,Peng J,Liu A,et al.Structure elucidation and NMRassignments of two new flavanones from the roots of Artocarpus heterophyllus[J].Magn Reson Chem,2015,53(10):872-874.
【关闭】
