期刊导航

论文摘要

不同细菌密度的浮游牙龈卟啉单胞菌及其生物膜对甲硝唑敏感性的体外研究

Susceptibility of Porphyromonas gingivalis to metronidazole at different planktonic cell densities and in biofilm

作者:段丁瑜 王爽 张利平 赵蕾 徐屹

Author:Duan Dingyu1, Wang Shuang1, Zhang Liping1, Zhao Lei1,2, Xu Yi1,2

收稿日期:2011-12-25          年卷(期)页码:2011,29(06):571-571-575

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:甲硝唑,牙龈卟啉单胞菌,生物膜,细菌密度,

Key words:metronidazole,Porphyromonas gingivalis,biofilm,cell density,

基金项目:

国家自然科学基金资助项目(30872872,30801295)

中文摘要

目的比较体外培养的牙龈卟啉单胞菌在不同细菌密度的浮游状态及生物膜状态时对甲硝唑的敏感性变化。初步探讨细菌密度在牙龈卟啉单胞菌生物膜抗药机制中的作用。方法采用微量液体稀释法,测定甲硝唑对牙龈卟啉单胞菌106CFU·mL-1和109CFU·mL-1菌悬液(与生物膜细菌密度相同)的最低抑菌浓度(MIC)和最低杀菌浓度(MBC);在96孔板中形成牙龈卟啉单胞菌生物膜,分别测定甲硝唑对完整生物膜及生物膜悬液的MIC和MBC。结果甲硝唑对106CFU·mL-1菌悬液的MIC和MBC分别为0.063、0.125 mg·L-1;对109 CFU·mL-1菌悬液的MIC和MBC分别为25、50 mg·L-1;对完整生物膜的MIC为25 mg·L-1,MBC>125 mg·L-1;对生物膜悬液的MIC和MBC分别为25、125 mg·L-1。结论甲硝唑对牙龈卟啉单胞菌的抑菌作用受细菌密度影响,细菌密度越高,对甲硝唑的抗性越大;细菌密度在牙龈卟啉单胞菌生物膜对甲硝唑的抗药机制中起到重要作用,但生物膜中胞外基质及生物膜的完整性对生物膜抗药性仍具有重要作用。

英文摘要

Objective To compare the susceptibility of Porphyromonas gingivalis to metronidazole at different planktonic cell densities and in biofilm, and to evaluate the role of cell density in antibiotic drug resistance in Porphyromonas gingivalis biofilm. Methods The minimum inhibitory concentration(MIC) and the minimum bactericidal concentration( MBC) of metronidazole against Porphyromonas gingivalis were detected by a broth dilution method under a final inocula of 106 CFU·mL-1 and 109 CFU·mL-1(cell number equal to biofilm). After the initial biofilm formed in the microtiter plate wells, the MIC and MBC of metronidazole to the intact and succedent resuspended biofilm were determined. Results The MIC and MBC of metronidazole against 106 CFU·mL-1 planktonic Porphyromonas gingivalis were 0.063, 0.125 mg·L-1 respectively. However, those against 109 CFU·mL-1 planktonic Porphyromonas gingivalis were 25, 50mg·L-1. Against intact Porphyromonas gingivalis biofilm, the MIC was 25mg·L-1 and MBC was higher than 125mg·L-1, those against resuspended biofilm was 25, 125 mg·L-1 respectively. Conclusion The resistance of Porphyromonas gingivalis to metronidazole increases along with the augment of the bacterial density. Cell density plays an important role in the resistance of biofilm. However, extracellular matrix and the integrity of biofilm may be the other influence factors for the biofilm resistance.

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