Objective The purpose of this study is to find some appropriate treatments on the dentin of root canal wall to obtain better bonding strength of fiber posts. Methods 36 new extracted non-caries single root canal anterior teeth were collected and post spaces were prepared. The teeth were divided into six groups randomly and processed by different post-space treatments: Sodium chloride irrigation(groupⅠ, control group); irrigation with 2% chlorhexidine solution followed by 17% EDTA solution(groupⅡ); irrigation with 3% hydrogen peroxide followed by 17% EDTA solution(group Ⅲ); irrigation with 5.25% sodium hypochlorite(NaClO) followed by 17% EDTA solution(group Ⅳ); etching with 35% phosphoric acid-gel for 30 s(group Ⅴ); 17% EDTA-gel for 1 min(group Ⅵ). After each post-space treatment, sodium chloride irrigation was applied in Ⅱ~Ⅵ group. Fiber posts were then luted in the treated roots using 3M ESPE RelyXTM Unicem and the thin-slice push-out test was performed. The dentin surfaces and bonding interface were examined under scanning electron microscope. Fracture mode of fiber posts were observed by stereo microscope. Results Group Ⅱ~Ⅵ were effective in getting rid of smear layer. Resin rags of dentin bonding interface were observed in group Ⅱ and group Ⅴ. The majority fracture mode of specimens in each group were mixed mode which contained dentin destruction. Compared with control group, the bonding strength of group Ⅱ , Ⅴ increased obviously(P