Objective To investigate the ectopic osteogenesis potential of human natural bone derived material combined with human bone marrow mesenchymal stem cells（MSCs）. Methods Cell-scaffold complexes were implanted subcutaneously into the left back of the nude mice, and human natural bone derived material were implanted into the right back as control group. The mice were killed respectively on the postoperative 2 weeks, 4 weeks and 8 weeks. The macroscopic, histopathological, alkaline phosphatase（ALP） activity assay methods were performed to assess the ectopic osteogenesis potential. Results The cartilaginous osteogenesis were observed in both deproteinated bone and decalcified bone，and the more new bone tissue formed gradually as the time went by after implantation. ALP activity become stronger followed with the time（P
