骨折愈合中纤维粘连蛋白和整合素β1的基因表达
Study on mRNA Expression of Fibronectin and Integrinβ1 During Fracture Healing
作者：刘少华,程刚,李声伟,田卫东,刘磊
Author：LIUShao-hua1,CHENGGang2,LI Sheng-wei3,TIANWei-dong3,LIULei3
收稿日期：2005-10-25 年卷（期）页码：2005,23(05):434-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：整合素β1,纤维粘连蛋白,骨折愈合,逆转录原位PCR,
Key words：integrinβ1,fibronectin,fracture healing,in situ RT-PCR,
基金项目：国家自然科学基金资助项目(39870750)
中文摘要
目的 探讨骨折愈合过程中纤维粘连蛋白(FN)和整合素β1 (Itgβ1)基因时空表达变化规律。方法 取兔
下颌骨骨折愈合各时期标本的石蜡切片,采用原位逆转录多聚酶链式反应技术,检测骨折愈合过程的不同时期FN
和Itgβ1 mRNA的时空表达。结果 ①骨折愈合过程中,FN mRNA和Itgβ1 mRNA在骨组织的多种细胞中广泛表
达,阳性信号出现于细胞浆和细胞核中;②整合素β1 mRNA在骨折5~7 d后表达增强,14~30 d最强,60~90 d时基
本接近正常;③FNmRNA骨折后3 d出现阳性表达,7~14 d达到峰值,然后急剧下降,30 d时有微弱表达,60 d后未
检测出表达。结论 骨折愈合过程中局部FNmRNA和Itgβ1 mRNA表达均增强,在时空关系上具有协同作用,提示
FN和Itgβ1对骨折愈合具有重要影响。
英文摘要
Objective To investigate the spatial and temporal expression changes of fibronectin and integrinβ1 mRNA during fracture healing.Methods Using in situ RT-PCR technique, the spatial and temporal expression pattern of both fibronectin and integrinβ1 mRNAwas detected on paraffined slices in different rabbit mandibular fracture healing phases.Results ①The mRNA of integrinβ1 and fibronectin was widely expressed in fractured bone cells. The positive stain existed in cytoplasm and nucleolus. ②After 7 days of fracture, increaseded integrinβ1 mRNA expression was observed, and it reached maximal levels approximately 14~30 days post-fracture and returned to normal levels after 60~90 days.③Fibronectin mRNA was detected on 3 days post- fracture, it reached maximal levels at 7~14 days,faint expressionwas detected at 30 days, undetectable 60 days afterwards.Con- clusion During fracture healing, the mRNA expression of integrinβ1 and fibronectin increased locally, FN and Itgβ1 cooperate with each other,it is suggested that they have an important influence on fracture healing.
上一条：口腔鳞癌中环氧合酶-2的表达与微血管密度的关系
【关闭】
