五倍子与氟化钠对人工口腔生物膜细菌生长代谢的影响
Comparison ofGalla Chinesisand Sodium Fluoride on Growth and Metabolism of Bacteria in Biofilm Model
作者：朱昞,李继遥,黄正蔚,李红,周学东
Author：ZHUBin-g1,LIJi-yao2,HUANG Zheng-wei1,LIHong1,ZHOUXue-dong2
收稿日期：2005-10-25 年卷（期）页码：2005,23(05):367-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：五倍子,氟化钠,抗菌作用,生物膜,
Key words：Galla Chinesis,sodium fluoride,antibacterial effect,biofilm,
基金项目：
国家自然科学基金资助项目(30171175);四川省教育厅国际合作项目基金资助项目(413020)
中文摘要
目的 探讨天然药物五倍子替代氟化物防龋的可能性。方法 以变形链球菌、血液链球菌、唾液链球菌和内氏放线菌为实验菌株,建立细菌生物膜模型。经五倍子和氟化钠作用后,通过对羟磷灰石片(HA)表面细菌计数,连续pH监测,生物膜超微结构观察,比较2种药物对生物膜细菌生长、产酸和生物膜表面形态的影响。结果 五倍子和氟化钠对生物膜中实验细菌的生长均有不同程度的抑制作用。五倍子和氟化钠均能抑制实验细菌产酸, 减少pH的下降,使终末pH值高于临界pH值,但五倍子的作用弱于氟化钠。扫描电镜下五倍子组和氟化钠组HA 表面均能形成典型的生物膜,细菌间界限清晰,细胞外基质形成减少,两组间差异无统计学意义。结论 天然药物五倍子能够有效抑制口腔生物膜细菌的生长代谢,其防龋效果可以肯定,值得进一步的研究开发。
英文摘要
Objective To compare the antibacterial effect between sodium fluoride andGalla Chinesisin biofilm model. To evaluate the feasibility of application ofGalla Chinesisin the clinical practice of caries prevention.Methods Streptococcus mu- tans,Streptococcus sanguis,Streptococcus salivariusandActinomyces naeslundiihad been chosen as the experimental bacteria. In the experiment, biofilmmodelwas constructed, and two agentswere added in the media. After a period of continuous culture, the number of bacteria adhering on the HA disc was examed, the planktonic pH in the flow cell was recorded continuously, and the morphology of the biofilmformed on theHAdisc was observed by SEM.Results ①Galla Chinesiscould inhibitthe growth of the four oral bacteria in the biofilm just as sodium fluoride.②Galla Chinesisand sodium fluoride could prevent the descent of the planktonic pH in the flow cell,butGalla Chinesiswas less efficient than sodium fluoride.③The biofilms formed after application ofGalla Chinesisand fluoride, but the bacterial cells had less matrixthan those applicatedwith sucrose.Conclusion Galla Chi- nesisis one kind of effective cariostatic natural agents.
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