Objective To investigate the biological effects of the new nano-hydroxyapatite/polyamide 66 biological composites (nHA-PA66) on the dental pulp cells.Methods After interaction with the nHA-PA66 eluate, the growth, proliferation, and function of theinvitrocultured human dental pulp cellswere studied by cell culture technique, inverted phase-contrastmicroscope observation, MTT assay, flow cytometry, ALP activity assay and quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) analysis.Results The cultured pulp cells grewwell and showed nomorphological variation. Moreover, thismaterial had no negative effects on the proliferation, cell cycle, ALP activity and the expression of dentin sialophosphoprotein (DSPP) mR- NA of the pulp cells.Conclusion As a newnano-biomaterial, nHA-PA66 has good biocompatibilitytothe pulp cells, but no ob- vious bioactivity.
