甘草甜素对小鼠颌下腺纤维肉瘤移植瘤抑制作用的实验研究
Inhibition Effect of Glycyrrhizin on Transplanted Mandibular Gland Fibrosarcoma of Mice
作者：于华,葛淑芬,王延高
Author：YUHua1,GE Shu-fen2, WANG Yan-gao3
收稿日期：2004-12-25 年卷（期）页码：2004,22(06):452-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：甘草甜素,纤维肉瘤,移植瘤,巨噬细胞,流式细胞术,
Key words：glycyrrhizin,fibrosarcoma,transplanted-tumor,phagocytosis,flow cytometry,
基金项目：
国家教委留学回国人员科研基金资助项目[教外司(1994) 660号];辽宁省自然科学基金资助项目(9522082)
中文摘要
目的 探讨甘草甜素(GL)的抗肿瘤作用和机制。方法 用化学致癌剂诱发小鼠颌下腺纤维肉瘤,再将肿瘤接种到同种小鼠腋下,观察肿瘤生长曲线、抑瘤率及肿瘤细胞形态学改变以研究GL对纤维肉瘤的抑制作用,同时通过流式细胞仪对细胞周期的分析观察进一步研究和探讨GL的抗肿瘤作用机制。结果 GL对小鼠颌下腺纤维肉瘤移植瘤抑制作用的最适剂量为每20 g体重1·61 mg,剂量在每20 g体重3·22 mg以下时无明显的毒副作用。 GL能引起小鼠纤维肉瘤细胞形态学的改变,促进巨噬细胞对肿瘤的免疫抑制作用。细胞周期解析的结果表明,GL 作用的纤维肉瘤细胞从DNA合成前期向DNA合成期移行受阻。结论 GL对小鼠颌下腺纤维肉瘤移植瘤有抑制作用,其作用机制可能与促进巨噬细胞对肿瘤的免疫抑制作用以及阻断DNA合成酶的作用有关。
英文摘要
Objective To study glycyrrhizin′s anticancer effect and its mechanism.Methods 3-methylcholanthrene were in- jected into mice′s submandibular glands to induce tumor, then transplanted the tumor pieces(1 mm3) to mice. The transplanted tumors were measured, and flow cytometry analysis and cytomorphology observation were conducted.Results Glycyrrhizin (GL) inhibited the transplanted mandibular gland fibro-sarcoma of mice and the suitable GL dose for inhibiting fibrosarcoma of mice was 1.61 mg per20 gweight. The GLdose below3.22 mg per20 gweight didn′t produce remarkable toxicity and side effects. GL in- duced cytomorphological changes of tumor cells and enhanced immunosuppression of macrophage on fibrosarcoma. The result of flow cytometry showed that tumor cell counts of GL1 and GL2 groups increased remarkably in DNA synthetic prophase, and de- creased in DNA synthetic phase.Conclusion GL can inhibit transplanted mandibular gland fibro-sarcoma of mice. The antican- cer mechanism of GLmay be acting on related enzymes with phagocytosis. The result of flow cytometry showed that the shift of fi- brosarcoma cells fromG1 phase to S phase was blocked. This suggests that the anticancer action of GL is related to its inhibition of ribonucleotide reductase, a rate-limiting enzyme in DNA synthesis.
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