血管生成抑制剂与化疗联合对裸鼠腺样囊性癌移植瘤生长的影响
Combining of TNP-470 and 5-Fu in Inhibition of Adenoid Cystic Carcinoma in Nude Mice Model
作者：农晓琳1,王大章2,蒙 敏1,周 诺1,蒙 宁1,李佳荃3,张 宏4
Author：NONG Xiao-lin1, WANG Da-zhang2,MENGMin1,ZHOUNuo1,MENGNing1,LIJia-quan3,ZHANGHong4
收稿日期：2004-08-25 年卷（期）页码：2004,22(04):267-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：腺样囊性癌,血管生成抑制剂,动物实验,
Key words：adenoid cystic carcinoma,angiogenesis inhibitor,animal experiment,
基金项目：
国家自然科学基金资助项目(30060082);广西教育厅资助项目(97095);广西卫生厅重点项目(重200006);教育部春晖计划启动基金项目(教外司留[2003]593)
中文摘要
目的 探讨单独使用血管生成抑制剂及其与化疗药联合对腺样囊性癌裸鼠移植瘤生长的影响。方法 BABL/C nu/nu裸小鼠接种腺样囊性癌Acc-M细胞系48 h后分6组:对照组;单纯皮下注射TNP-470低、中、高剂量组;单纯腹腔注射5-Fu组;中剂量TNP-470加5-Fu联合治疗组,分别用药治疗,观察治疗效果。瘤组织标本行血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)免疫组化染色及透射电镜观察。结果 TNP-470 100 mg/kg 组,TNP-470 30 mg/kg与5-Fu 50 mg/kg联合使用组有显著抑瘤效果;电镜下见凋亡瘤细胞增多。各实验组均有 VEGF、bFGF的不同程度表达。结论 血管生成抑制剂可抑制腺样囊性癌生长,但因其生长相对缓慢,敏感性不及肉瘤等生长快、血供丰富的肿瘤明显,与化疗药联合使用可达到较好的协同作用。
英文摘要
Objective To study the effect of angiogenesis inhibitor and its combine with chemical drug in suppressing the growth of adenoid cystic carcinoma (ACC).Methods Acc-M cellswere inoculated subcutaneous into BABL/C nu/nu mice. The mice were divided into control, different dose of TNP-470 treatment groups, 5-Fu treatment group and TNP-470 plus 5-Fu treat- ment group. Treatments were given 48 hours after inoculation. The mice were sacrificed on the 22nd day and excised tumors were weighted. Tumors were also investigated by immunohistochemistry and ultrastructural observations.Results TNP-470 100 mg/kg/qod efficiently inhibited the growth of Acc-Mtumors. TNP-470 30 mg/kg/qod combined with 50 mg/kg/week 5-Fu also resulted in significant growth inhibit of the tumors. TNP-470 suppressed tumor growth by inhibiting neovascularization, therefore inducing apoptosis of Acc-M cells. All experimental groups had different degrees of VEGF and bFGF express.Conclusion Since ACC is a slow developing tumor, blood supply is not so sufficient as sarcomas. Angiogensis inhibitormay inhibit its growth in high dosage. Combining medium dosage of angiogensis inhibitor with chemical drug may have synergistic result in inhibiting ACC growth.
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