Objective The micronucleus test was applied to evaluate the genotoxicity of a new nanocomplex HA-PA66 root filling materialin vitro.Methods The dulbecco′s modified eagle media(DMEM) extracts of the powder part and the mixture of the new nanomaterial were prepared separately. The V79 cellwas used as the test cell and the mitomycin C(MMC) as the positive control. The MTT assay was employed in our study to evaluate the cytotoxic effect while the number of micronucleus was used as the criteria forthe detection of genotoxocity.Results TheMTTvalues in test groups and negative groupwere not significantly dif- ferent at different times (P>0.05). The number of micronucleus in test groups (powder group: 6.1±1.1/1 000; complex group: 5.7±0.6/1 000) was similar to the negative control(5.3±0.8/1 000,P>0.05), while theywere significantly different to the positive control(123.9±8/1 000,P
