葫芦素BE聚乳酸纳米微粒对口腔癌颈淋巴结转移灶靶向治疗的应用研究
Clinical Application of Anticancer Nanoparticles Targeting Metastasis Foci of Cervical Lymph Nodes in Patients with Oral Carcinoma
作者：杨凯,温玉明,王昌美,郑刚
Author：YANGKai*,WENYuming,WANGChangmei,et al.
收稿日期：2003-12-25 年卷（期）页码：2003,21(06):447-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：聚乳酸,纳米粒,口腔肿瘤,淋巴结转移,靶向治疗,
Key words：polylactic acid,nanoparticles,oral neoplasm,lymph nodes metastasis,targeted therapy,
基金项目：
本课题为卫生部科学研究基金(编号98-1-221)、四川省卫生厅科学研究基金(编号F981044)和重庆医科大学附属第一医院博士科研启动基金(编号2002007)资助项目
中文摘要
目的 评价平均粒径85 nm的抗癌葫芦素BE聚乳酸纳米微粒(CuBE-PLA-NP)口腔癌周黏膜下注射对颈淋巴结转移灶的靶向性和治疗效果。方法 26例口腔鳞癌患者分为实验组和对照组。实验组用 CuBE-PLA-NP于术前2、12、24、48、72、96、120、144、168、192 h和对照组于术前2、12、24 h用葫芦素BE (CuBE)行癌周黏膜下注射。然后用高效液相色谱仪检测两组患者各时间点颈淋巴结内和血中药物浓度,并用光镜和电镜观察颈淋巴结转移灶内癌细胞的坏死情况。结果 (1)实验组各时间点颈淋巴结中药物浓度远远高于对照组,且持续时间明显长于对照组。(2)实验组各时间点血中药物浓度显著低于对照组。(3)实验组颈淋巴结内可见明显的癌细胞坏死、变性,而对照组颈淋巴结内无癌细胞变性、坏死。结论 平均粒径为85 nm的CuBE-PLA-NP对口腔癌颈淋巴结转移灶具有良好的靶向性,达到了提高颈淋巴结转移灶内药物浓度和延长药物持续时间,提高了疗效,同时降低了药物的全身毒副作用。
英文摘要
Objective The aim of this studywas to investigate the target delivery of Cucurbitacin BE (CuBE) to cervical lymph nodes by peri-oralcancer submucosal injection of the average diameter 85nm Cucurbitacin BE polylactic acid nanoparticles (CuBE-PLA-NP) and evaluate its clinical therapy efficacy.Methods CuBE and CuBE-PLA-NP were respectively injected into perioralcancer submucosa at 2、12、24、48、72、96、120、144、168 and 192 hours before operation in 26 patients with oral cancer. The concentrations of CuBE in cervical lymph nodes and bloodwere measured by high performance liquid chromatography (HPLC) at every time point. Cancer cell degeneration and necrosis in metastasis foci of cervical lymph nodeswere observed using light mi- croscope and electron microscope.Results (1)The CuBE concentrations in cervical lymph nodes after CuBE-PLA-NP injection were far higher than those after CuBE injection at every time point, the duration of CuBE existing in the cervical lymph nodes in CuBE-PLA-NP group was far longer than those in CuBE group, the area under the CuBE concentrations-time curve of the cervical lymph nodes in CuBE-PLA-NPgroupwas 43.67 times asmany as that in CuBEgroup; (2)The CuBE ratio ofmaximum concentra- tions in the cervical lymph nodes in CuBE-PLA-NP group was 106.46 times as high as that in CuBE group; (3) The CuBE con- centrations in the blood in CuBE-PLA-NP groupwas far lower than that in CuBE group; (4)Cancer cell necrosis and degeneration in the metastasis foci of cervical lymph nodes were found in CuBE-PLA-NP group, necrosis and degeneration were not found in CuBE group.Conclusion The perioralcancer submucosa injection of the average diameter of 85nm CuBE-PLA-NP can specifical- ly deliver CuBE to the cervical lymph nodes, enhance treatment efficiency, and reduce general toxicity.
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