3种寡核苷酸探针对龈下菌斑中牙周致病菌的检测

Distribution of 3 Kinds of Periodontal Pathogens in Subgingival Plaques of Patients with Chronic Periodontitis

作者：张贤华,张斌,吴织芬

Author：ZHANG Xianhua*,ZHANGBin,WUZhifen.

收稿日期：2003-08-25 年卷（期）页码：2003,21(04):287-

期刊名称：华西口腔医学杂志

Journal Name：West China Journal of Stomatology

关键字：牙周炎,牙龈卟啉单胞菌,福赛类杆菌,牙密螺旋体,寡核苷酸探针,

Key words：periodontitis,Porphyromonas gingivalis,Treponema denticola,Bacteridesforsythus,oligonucleotide DNA probe,

基金项目：

中文摘要

目的　采用寡核苷酸探针研究龈下菌斑中3种牙周致病菌的分布。方法　利用3种寡核苷酸探针对 60例慢性牙周炎患者60个患病位点、10例健康人的10个健康对照位点龈下菌斑中的牙龈卟啉单胞菌、福赛类杆菌、牙密螺旋体进行检测。结果　牙周炎位点龈下菌斑中的牙龈卟啉单胞菌、福赛类杆菌、牙密螺旋体的检出率分别为91·67%,90·00%和95·67%,明显高于健康对照位点;有83·33%的牙周炎位点同时检出3种致病菌,3种细菌检出情况为两两正相关(P

英文摘要

Objective To observe the prevalence ofPorphyromonas gingivalis(Pg),Treponema denticola(Td) andBac- teridesforsythus(Bf) subgingivally in diseased sites of chronic periodontitis (CP) patients.Methods Samples of subgingival plaque were detected by 3 kinds of oligonucleotide DNA probe from 60 sites of CP patients and 10 healthy sites of 10 healthy peo- ple.Results The positive rates ofPg,BfandTdin patients were 91·67%, 90·00% and 95·67% respectively;Pg,Bfand Tdwere detected simultaneously in 83·33% patients andPg,BfandTdwere found to be related with each other(P

【关闭】

论文摘要

3种寡核苷酸探针对龈下菌斑中牙周致病菌的检测

Distribution of 3 Kinds of Periodontal Pathogens in Subgingival Plaques of Patients with Chronic Periodontitis