颊、腭粘膜移植修复硬腭裸露骨面的组织学观察
A Histological Study on Healing Process of Palatal Wound withDenuded Bone Restored with Transplanted Buccal or Palatal Mucosa
作者：王志勇,石冰,鲁大为,宋庆高
Author：Wang Zhiyong, Shi Bing, Lu Dawei, Song Qinggao
收稿日期：2002-10-25 年卷（期）页码：2002,20(05):326-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：唇腭裂,颊粘膜,腭粘膜,裸露骨面,组织学,
Key words：cleft palate and lip,buccal mucosa,palatal mucosa,denuded bone,histology,
基金项目：
本课题为高等学校优秀教师教学和科研奖励基金(1999)和霍英东青年教师基金(编号71040)资助项目
中文摘要
目的 :研究硬腭裸露骨面和应用颊、腭粘膜移植覆盖裸露骨创面后的组织学愈合过程。方法 :选用 32只日本大耳白纯种兔 ,随机分为正常对照组、裸露骨面组、颊粘膜移植组、腭粘膜移植组。裸露骨面组 ,用外科手术形成一恒定大小的矩形裸露骨面 ;颊、腭粘膜移植组 ,则在裸露骨面的基础上 ,分别用相同面积的颊、腭粘膜移植覆盖裸露骨面。术后定期处死动物 ,切取硬腭制作组织学切片 ,观察不同处理措施的腭部组织学愈合过程。结果 :裸露骨面组 ,术后 2周出现致密纤维结缔组织 ;术后 4周 ,软组织与骨组织之间出现Sharpey′s纤维 ,并存在至观察期结束。采用颊、腭粘膜移植组 ,结缔组织较疏松 ,无Sharpey′s纤维形成 ,组织结构类似于正常组织。结论 :采用颊、腭粘膜移植修复硬腭裸露骨面 ,可有效地阻止Sharpey′s纤维形成。
英文摘要
Objective:The purpose of this study was to observe the healing process of palate wound with denuded bone restored with transplanted buccal or palatal mucosa and to elucidate the mechanism of maxillary growth inhibition following palate repair.Methods: 32 Japan white rabbits, 5 weeks old, were selected as the subjects for this study. They were divided into 4 groups at random. The rabbits in group I was the control without receiving any treatment. The rabbits in group II, III, IV was surgically denuded the bone of palate, and afterwards, the rabbits in group II were not received further restoration, but rabbits in group III and IV were restored with transplanted buccal and palatal mucosa respectively. From 2 to 14 weeks after surgery, at regular intervals, palatal wounds were observed by using a light microscope. Histological changes were also compared among different groups.Results: It was found in group II that dense connective tissue was formed 2 weeks after the surgery, and Sharpey′s fibers was formed between the scar and bone tissue 4 weeks after the surgery. However, no Sharpey′s fiber was found in group III and group IV, and in the latter two groups, the histological character of tissue was similar to that of the control.Conclusion: Prevention of the attachment of Sharpey′s fibers to the palatal bone could be effectively accomplished by covering the denuded palatal bone with the transplanted buccal or palatal mucosa.
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