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论文摘要

Nd: YAG激光对牙根面结构及变异链球菌黏附的影响

Effects of Nd: YAG laser irradiation on the root surfaces and adhesion of Streptococcus mutans

作者:李圆红,李忠成,罗梦奇,申道南,张舒,孟姝

Author:Li Yuanhong, Li Zhongcheng,Luo Mengqi, Shen Daonan, Zhang Shu, Meng Shu

收稿日期:2016-04-11          年卷(期)页码:2016,34(6):579-579-583

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:Nd: YAG激光,变异链球菌,根面,

Key words:Nd: YAG laser,Streptococcus mutans,root surfaces,

基金项目:

中文摘要

目的 研究不同功率的水冷Nd: YAG激光照射治疗对牙根面结构及变异链球菌黏附的影响。方法 将因重度慢性牙周炎拔除的离体牙分为4组,均进行手工刮治和根面平整,制备牙根片,激光1、2、3组分别用功率为4、6、8 W的水冷Nd: YAG激光照射处理60 s,对照组不作处理。用扫描电子显微镜(SEM)观察各组根片的表面结构。将变异链球菌接种于各组牙根片表面并进行培养,计数各组根片表面细菌的黏附量(CFU·mL-1);同时用SEM观察对照组及激光2组根面的细菌黏附情况。结果 SEM观察根片表面结构,激光组较对照组牙根表面的玷污层、碎屑及菌斑样物质附着减少,但裂隙增多。3个激光组牙根表面的细菌黏附量均少于对照组(PP>0.05)。SEM观察细菌黏附情况,激光2组表面的细菌黏附量明显少于对照组。结论 与单纯刮治相比,刮治后进行水冷Nd: YAG激光治疗能清除牙根表面的玷污层、碎屑及菌斑样物质,并能减少变异链球菌的黏附,但激光会导致牙根表面出现裂隙。本实验中,6 W为激光最佳功率,既能较好地去除牙根表面的玷污层和碎屑等,又能较好地控制激光对根面的热损害程度。

英文摘要

Objective This study aimed to evaluate the effects of treatment with different powers of Nd: YAG laser irradiationon root surfaces andStreptococcus mutans(S. mutans) adhesion. Methods Extracted teeth because of severe periodontaldisease were divided into the following four groups: control group, laser group 1, laser group 2, and laser group 3. Afterscaling and root planning, laser group 1, laser group 2, and laser group 3 were separately treated with Nd: YAG laser irradiation(4/6/8 W, 60 s); however, the control group did not receive the treatment. Scanning electron microscopy (SEM) was used todetermine the morphology.S. mutanswere cultured with root slices from each group. Colony forming unit per mL (CFU·mL-1)was used to count and compare the amounts of bacteria adhesion among groups. SEM was used to observe the difference ofbacteria adhesion to root surfaces between control group (scaling) and laser group 2 (6 W, 60 s), thereby indicating the differentbacteria adhesions because of different treatments. Results Morphology alterations indicated that root surfaces in controlgroup contain obvious smear layer, debris, and biofilm; whereas the root surfaces in laser group contain more cracks withless smear layer and debris. The bacteria counting indicated thatS. mutansadhesion to laser group was weaker than that ofcontrol group (PP>0.05) was observed. Morphology alterationsalso verified thatS. mutansadhesion to laser group 2 (6 W, 60 s) was weaker than that of control group (scaling). ConclusionThis study demonstrated that Nd: YAG laser irradiation treatment after scaling can reduce smear layer, debris, and biofilmon the root surfaces as compared with conventional scaling. The laser treatment reduces the adhesion ofS. mutansas well.However, Nd: YAG laser irradiation can cause cracks on the root surfaces. In this experiment, the optimum laser power of 6 W thoroughly remove the smear layer and debris, as wellas relatively improve the control of thermal damagee.

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