靶向舌癌多功能纳米粒的制备和体外实验
Preparation of multifunctional nanoparticles targeting tongue cancer and in vitro study
作者：任为, 邱丽华, 高志, 李攀, 赵欣, 胡程晨
Author：Wei Ren, Lihua Qiu, Zhi Gao, Pan Li, Xin Zhao, Chengchen Hu
收稿日期：2017-11-14 年卷（期）页码：2018,36(3):240-240-246
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：聚乳酸-羟基乙酸共聚物,吲哚菁绿,靶向治疗,舌癌,分子成像,
Key words：poly (lactic-co-glycolic acid),indocyanine green,targeted therapy,tongue carcinoma,molecular imaging,
基金项目：重庆市社会民生科技创新专项项目（cstc2016shmszx000-10）;2016年重庆高校创新团队建设计划资助项目（CXTDG20160-2006）;重庆市高校市级口腔生物医学工程重点实验室资助项目[渝教科（2014）55号]
中文摘要
目的 制备一种基质细胞衍生因子-1（SDF-1）修饰的载多西紫杉醇（DOC）包裹吲哚菁绿（ICG）和液态氟碳全氟己烷（PFH）靶向多功能纳米粒（DOC-SINPs）,检测其性质。方法 双乳化法制备纳米粒,硫醚键连接SDF-1,得到靶向纳米粒。Malvern粒径仪检测其粒径及表面电位,激光扫描共聚焦显微镜观察SDF-1在纳米粒表面的连接情况,高效液相色谱法（HPLC）测定其DOC包封率（ELC）和载药量（DLC）及体外释放规律,热成像仪记录其体外光热特性,光声仪及超声诊断仪观察其体外显像,流式细胞仪评估其体外靶向能力,CCK-8法检查其对舌癌SCC-15细胞活力的影响。结果 靶向多功能纳米粒形态规则,呈球状。平均粒径（502.88±17.92） nm,平均电位（-11.5±3.15） mV。平均ELC为54.12%±1.74%,平均DLC为1.08 mg·mL-1。体外药物释放规律呈初期爆发性释放,接着是持续缓慢释放。其光热特性呈浓度相关,浓度越高温度越高。纳米粒可检测到明显光声信号和超声信号。体外靶向连接率89.99%。细胞活性实验结果表明,SINPs组在各个浓度下细胞活性无明显差异（P>0.05）。在浓度为150、200 μg·mL-1时,DOC-SINPs无论有无激光辐照均较SINPs有明显的细胞活性抑制（P<0.05）。结论 成功制备了集诊断与治疗一体的多功能纳米粒,具有超声/光声双模成像,同时具备化疗和光热治疗的抗肿瘤作用。
英文摘要
ObjectiveThis study aims to prepare docetaxel (DOC)-loaded multifunctional nanoparticles containing indo-cyanine green (ICG) and perfluorohexane (PFH) as targeted drug delivery system, which is supplemented with stromal cell-derived factor-1 (SDF-1), and characterize their properties.MethodsMultifunctional nanoparticles were prepared by using the double emulsion method. SDF-1 was covalently conjugated to the surface of the nanoparticles through thioe-ther bonding. Their particle size, distribution, and surface potential were determined with the Malvern measuring in-strument. The conjugation of SDF-1 was evaluated by confocal laser scanning microscope. Encapsulation efficiency (ELC), drug loading capacity (DLC), and release regularity of the nanoparticles were determined by high-performance liquid chro-matography (HPLC).In vitrophotothermal property was recorded by a thermal imager. Thein vitroimaging capacity was observed by a photoacoustic instrument and an ultrasonic diagnostic apparatus. Targeting capability was assessed by flow cytometry. The cell activity on SCC-15 cells was checked by CCK-8 method.ResultsThe targeted multifunctional nano-particles showed regularly sphericity. The diameter was (502.88±17.92) nm. The zeta potential was (-11.5±3.15) mV. ELC was 54.12%±1.74%. DLC was 1.08 mg·mL-1.In vitrodrug release was initially fast and subsequently slow. The photothermal characteristics were related to the concentration; the higher the concentration, the higher the temperature. Nanoparticles could detect significant photoacoustic and ultrasound signals. Thein vitrotargeting rate was 89.99%. No significant differences of cell viability in the SINPs groups were observed at each concentration (P>0.05). The inhibition effect of DOC-SINPs was stronger than that of SINPs whether or not in the presence of laser irradiation among the groups of 150 and 200 μg·mL-1(P<0 .05).ConclusionMultifunctional nanoparticles for diagnosis and treatment were successfully prepared and displayed dual-mode ultrasound/photoacoustic imaging and antitumor effects of chemotherapy and photothermal therapy.
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