细胞内渗透压改变对三叉神经电压门控钠离子通道电流的影响
Effects of intracellular osmolality changes on the voltage-gated sodium channels currents of trigeminal ganglion neuron
作者：沈颉飞 王海业 麻颖宜 刘云飞 张书垣 杜莉
Author：Shen Jiefei, Wang Haiye, Ma Yingyi, Liu Yunfei, Zhang Shuyuan, Du Li.
收稿日期：2011-08-09 年卷（期）页码：2012,30(4):338-338-342
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：三叉神经节神经元, 渗透压浓度, 电压门控钠离子通道, 电生理特性,
Key words：trigeminal ganglion neuron, osmolality, voltage-gated sodium channels, electrophysiology,
基金项目：
国家自然科学基金青年科学基金资助项目（81000456）；四川省科技支撑计划基金资助项目（2010SZ0100）
中文摘要
目的探讨细胞内液渗透压对三叉神经节神经元（TRGN）上的电压门控钠离子通道（VGSCs）的生物力学影响。方法TRGN细胞从SD乳鼠分离出来之后，运用膜片钳全细胞记录的方法对VGSCs电流进行记录和分析，通过调节电极内液的组成成分来控制细胞内液渗透压；再与正常渗透压相对比，探讨低渗（260 mOsm）和高渗（350 mOsm）对通道激活和失活动力学的影响。结果与对照组相比较，细胞内液低渗刺激可以影响VGSCs电流的激活和失活特性，包括V0.5、失活曲线（G-V曲线）特征均有明显的统计学意义（P
英文摘要
Objective To evaluate the biomechanical effects of intracellular changes on the voltage-gated sodium channels（VGSCs） on trigeminal ganglion neuron（TRGN）. Methods TRGN cells were acutely isolated from the neonatal SD rats. The voltage-dependent currents of the VGSCs on these neurons were elicited and analyzed by whole-cell patch-clamp recordings and the intracellular anisotonicity stimuli was established by adjusting the content of pipette solution. The effects of hypo-（260 mOsm） and hypertonic（350 mOsm） osmolarity on the activation and inactivation kinetics of VGSCs on TRGN were evaluated, compared with the normal intracellular environment. Results The results demonstrated that intracellular hypotonic stimuli could influence both the activation and inactivation characteristics of VGSCs currents, including the membrane potential at half inactivation（V0. 5） of the G-V and inactivation curves had obvious statistics significance（P
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